中国畜牧兽医2017，Vol.44Issue(5)：1281-1288,8.DOI:10.16431/j.cnki.1671-7236.2017.05.004
siRNA及其运载系统研究进展
Research Progress on Small Interfering RNA and Its Delivery Systems
摘要
Abstract
Small interfering RNA (siRNA)has proved to be a powerful tool for target-specific gene silencing via RNA interference (RNAi).Its ability to control targeted gene expression gives new hope to gene therapy as a treatment for cancers and genetic diseases.However,siRNA shows poor pharmacological properties,such as low serum stability,off-targeting,and innate immune responses,which present a significant challenge for clinical applications.In addition,siRNA can-not cross the cell membrane for siRNA activity because of its anionic property and stiff structure. Therefore,the development of a safe,stable,and efficient system for the delivery of siRNA thera-peutics into the cytoplasm of targeted cells is crucial.Several nanoparticle platforms for siRNA delivery have been developed to overcome the major hurdles facing the therapeutic uses of siRNA. This review covers a broad spectrum of non-viral siRNA delivery systems developed for enhanced cellular uptake and targeted gene silencing in vitro and in vivo ,and discusses their characteristics and opportunities for clinical applications of therapeutic siRNA,in order to lay a good foundation for the later research.关键词
siRNA/运载系统/基因沉默Key words
siRNA/delivery systems/gene silencing分类
生物科学引用本文复制引用
王洋,金一..siRNA及其运载系统研究进展[J].中国畜牧兽医,2017,44(5):1281-1288,8.基金项目
国家自然科学基金项目(3126052) （3126052）
吉林省科技厅重点攻关项目(20150204079NY) （20150204079NY）
