中国畜牧兽医2017，Vol.44Issue(5)：1289-1294,6.DOI:10.16431/j.cnki.1671-7236.2017.05.005
塔里木马鹿鹿茸VEGF基因克隆及其在不同鹿茸组织中的表达研究
Clone and Expression ofVEGF Gene in Different Tissue of Tarim Red Deer Antler
摘要
Abstract
This study was aimed to clone the coding sequence of vascular endothelial growth factor (VEGF)from the antler tissue at the top of the Tarim Red deer,and analyze the molecular prop-erties and expression in antler tissue.This study extracted total RNA in antler tip by improved Trizol method and got the VEGF gene by RT-PCR method,then purified and connected it to pMD18-T vector,and conversed it into Escherichia coli DH5α,the expression of the VEGF pro-tein at the top of antler tissue,between the cortex and chopped level of mesenchymal and carti-lage layer in Red deer were determined using immunohistochemical method.The results showed that the open reading frame (ORF)length of VEGF gene was 648 bp,and encoding 216 amino acids,sequence homology of the nucleotide were 98.75％,96.55％,97.58％ and 97.56％ com-paring with human,cattle,sheep and pig,respectively.The VEGF of Tarim Red deer antler was high similarity with that of human through its sequence analysis and comparison.The VEGF pro-tein were expressed in the top of antler tissue,including skin mesenchymal and cartilage layer from immunohistochemical experiment,but there was no obvious difference.It was predicted that the Tarim Red deer antler might be ideal model of the blood vessel related of regenerative medicine diseases for human research.关键词
塔里木马鹿茸/血管内皮生长因子/克隆/免疫组化Key words
Tarim Red deer antler/vascular endothelial growth factor/cloning/immunohisto-chemical分类
生物科学引用本文复制引用
刘俊峰,王晓婷,钟婷,郭雪峰..塔里木马鹿鹿茸VEGF基因克隆及其在不同鹿茸组织中的表达研究[J].中国畜牧兽医,2017,44(5):1289-1294,6.基金项目
兵团塔里木畜牧科技重点实验室项目(HS201509) （HS201509）
