中国畜牧兽医2017，Vol.44Issue(5)：1295-1301,7.DOI:10.16431/j.cnki.1671-7236.2017.05.006
鸡细小病毒半巢式PCR检测方法的建立及初步应用
Development and Preliminary Application of a Semi-nested PCR Assay for Detection of Chicken Parvovirus
摘要
Abstract
In order to set up and optimize a semi-nested PCR for rapid detection of chicken parvo-virus (ChPV),three specific primers were designed according to conserved sequences of NS 1 gene of ChPV.The specificity and sensitivity of ChPV semi-nested PCR were tested,and the as-say was applied to detect 48 clinical samples.The specificity and sensitivity tests showed that this semi-nested PCR was only sensitive to ChPV for amplifying specific band of 186 bp and it could detect 5.62 fg/μL of ChPV DNA,without any sensitivity to other viruses,such as Newcastle disease virus,H9 subtype avian influenza virus,Marek's disease virus,infectious laryngotrache-itis virus and infectious bronchitis virus.48 chicken samples were detected and the positive rate was 16.67％ (8/48).The results of our study demonstrated that the optimized semi-nested PCR could be a method that was suitable for clinical detection of ChPV.关键词
鸡细小病毒/半巢式PCR/检测Key words
chicken parvovirus/semi-nested PCR/detection分类
农业科技引用本文复制引用
奉彬,罗思思,邓显文,谢志勤,刘加波,宋中宝,林二克,谢芝勋,张艳芳,黄娇玲,王盛,范晴,黄莉,谢丽基,曾婷婷..鸡细小病毒半巢式PCR检测方法的建立及初步应用[J].中国畜牧兽医,2017,44(5):1295-1301,7.基金项目
广西科技项目(15-140-31-A-1、15-140-31-B-1、桂科AB16380003) （15-140-31-A-1、15-140-31-B-1、桂科AB16380003）
南宁市科技攻关项目(20152308) （20152308）
国家"万人计划"领军人才专项(2016-37-88) （2016-37-88）
