中国畜牧兽医2017，Vol.44Issue(5)：1302-1308,7.DOI:10.16431/j.cnki.1671-7236.2017.05.007
北京地区仔猪大肠杆菌血清型鉴定及其毒力因子检测
Detection of Serotypes and Virulence Factors of Escherichia coli from Piglets in Beijing
摘要
Abstract
To investigate the cause of piglets diarrhea,and the distribution of the serotype and virulence factors of swine Escherichia coli in Beijing,400 diarrhea samples were collected.TSA serum agar culture method was used to isolate Escherichia coli ,serotype identification test and virulence factor genes test were used to verify the presence of O-antigen.64 strains of E .coli were isolated from 400 diarrhea samples,among which 42 strains of E .coli were classified as 8 se-rotypes:O101(18.7％),O64(12.5％),O8 (10.9％),O20 (10.9％),O45 (4.7％),O149 (4.7％), O2(1.6％)and O89(1.6％),and the major virulence factors were STa,Stx2e,astA and eaeA. There were 8 mainly serotypes that caused piglets diarrhea in Beijing area,among which O101 se-rotype accounted for the highest proportion.The major virulence factors were astA and eaeA,ac-counted for more than 50％ of all strains,STa and Stx2e accounted for more than 30％ of all strains.These data would provide effective data to support the prevention and control of piglet di-arrhea in Beijing.关键词
仔猪腹泻病/大肠杆菌/血清型/毒力因子Key words
piglet diarrhea/Escherichia coli/serotype/virulence factor分类
农业科技引用本文复制引用
王振玲,段跃强,王金秋,王宇..北京地区仔猪大肠杆菌血清型鉴定及其毒力因子检测[J].中国畜牧兽医,2017,44(5):1302-1308,7.基金项目
北京市"菜篮子"科技能力提升工程项目 （）
北京农业职业学院院级项目 （）
