中国畜牧兽医2017，Vol.44Issue(5)：1309-1314,6.DOI:10.16431/j.cnki.1671-7236.2017.05.008
屎肠球菌荚膜多糖的提取纯化及其多糖含量的测定
Extraction,Purification and Determination of Capsular Polysaccharide in Enterococcus faecium
摘要
Abstract
In order to extract and purify the capsular polysaccharide of Enterococcus faecium and measure contents of polysaccharide,high pressure crushing methods and enzyme decomposition methods (RNase A (25 μg/mL),DNase (0.5 mg/mL),lysozyme (0.25 mg/mL)and protease K (1.25 mg/mL))were used to remove the nucleic acids and proteins to obtain crude capsular poly-saccharide,and centrifugal ultrafiltration with 30 and 100 ku ultrafiltration centrifuge tubes and DEAE Sepharose Fast Flow column chromatography were also adopted to obtain the purified cap-sular polysaccharides of Enterococcus faecium .Then the content of polysaccharide was determined by the phenol-sulfuric acid method.The results showed the capsular polysaccharide content was 75.21％.The results revealed that capsular polysaccharide of Enterococcus faecium was extrac-ted successfully.The method of extracting capsular polysaccharide in Enterococcus faecium was established initially.关键词
屎肠球菌/荚膜多糖/提取纯化/苯酚-硫酸法Key words
Enterococcus faecium/capsular polysaccharide/extraction and purification/phenol-sul-furic acid method分类
农业科技引用本文复制引用
刘永安,李学瑞,周建华,马丽娜,刘永生..屎肠球菌荚膜多糖的提取纯化及其多糖含量的测定[J].中国畜牧兽医,2017,44(5):1309-1314,6.基金项目
国家自然科学基金(31172335) （31172335）
