中国畜牧兽医2017,Vol.44Issue(5):1406-1413,8.DOI:10.16431/j.cnki.1671-7236.2017.05.022
延边黄牛黑皮质素受体-4基因多态性及其与肉质性状的相关性分析
The Correlation Analysis Between the SNPs of MC4R Gene and Meat Quality Traits in Yanbian Yellow Cattle
摘要
Abstract
In order to explore the effects of the key gene of regulating cattle fat deposition on meat quality traits,screening candidate gene which affect beef fattening traits.This study employed 157 cattles (24 months old)with similar weight as research object,analyzed the correlation be-tween SNPs of MC4R gene and meat quality traits in Yanbian Yellow cattle by PCR direct se-quencing method.The results indicated that the mutation site of C→G had been found in MC4R gene 1069 bp,which resulted in L (leucine)translated into V (valine).Protein secondary struc-ture lost α-spiral and β-fold at the 166,243 and 280 amino acid position,which resulted in the changes of spatial position ofβ-turns in tertiary structure.This site showed different degrees cor-relation with backfat thickness,lrightness,yellowness of Yanbian Yellow cattle.The backfat thickness and yellowness (3 d)of GG genotype were significantly higher than CC and CG geno-types (P <0.05).The lrightness of CC and CG genotypes were significantly higher than CC gen-otype (P <0.05).These results declared that the site had remarkably correlatived with Yanbian Yellow cattle relevant economic traits,and the mutation site could be used as a potential molecu-lar marker.关键词
延边黄牛/MC4R基因/SNPs/肉质性状Key words
Yanbian Yellow cattle/MC4R gene/SNPs/meat quality traits分类
农业科技引用本文复制引用
冯健,李英花,严昌国..延边黄牛黑皮质素受体-4基因多态性及其与肉质性状的相关性分析[J].中国畜牧兽医,2017,44(5):1406-1413,8.基金项目
吉林省科技发展计划项目(20140201004NY) (20140201004NY)