中国畜牧兽医2017，Vol.44Issue(5)：1468-1476,9.DOI:10.16431/j.cnki.1671-7236.2017.05.030
贵州某鸡场大肠杆菌与葡萄球菌混合感染病原的分离鉴定
Isolation and Identification of Pathogen of Escherichia coli and Staphylococcus Mixed Infection of Chichen in Guizhou Province
摘要
Abstract
In order to analyze the variety,drug resistance and pathogenicity of bacterial of a chick-en farm in Guizhou province,bacterial isolation culture was done from four sick chickens that were submitted by the farm.Gram staining,biochemical test,drug susceptibility test,16S rDNA sequencing analysis and so on were carried out to identify the bacteria.The results showed that we isolated two strains of bacteria with different colony morphology that one of them was gram-nega-tive bacilli,the other was gram-positive cocci, which were named as GZHX2016-1 and GZHX2016-2 according to the isolated location and time.GZHX2016-1 was Escherichia coli (E .coli)with multiple drug,and GZHX2016-2 was Staphylococcus simulans which was negative on Staphylococcus coagulase test and was resistant to some antibiotics.The 16S rDNA sequen-cing of GZHX2016-1 was more close genetic relationship with E .coli (GenBank accession No.:CP007442.1, CP014667, KT156725.1, and so on ), and the homology was 99.5％.The GZHX2016-1 was more close genetic relationship with Staphylococci (GenBank accession No.:HM140412.1,AM944030.1, KM877513.1, and so on ), and the homology was 97.9％. GZHX2016-1 and GZHX2016-2 were pathogenic to mouse.The minimum lethal dose of the GZHX2016-1 was 1.12 ×108 CFU of each mouse by intraperitoneal injection,and the minimum lethal dose of GZHX2016-2 was 3.20×107 CFU.In summary,the experiment successfully isola-ted a strain of pathogenic E .coli and a strain of coagulase-negative Staphylococcus from chick-ens.The chicken farm was infected by E .coli and Staphylococcus ,and the bacteria were resistant to multiple antibiotics.So,we should take effective measures to control the bacteria pollution in the chicken farm,and restrict the use of antibiotics to reduce the resistance bacteria,and ensure the food safety of chicken and chicken products.关键词
模仿葡萄球菌/大肠杆菌/生化鉴定/16SrDNAKey words
Staphylococcus simulans/Escherichia coli/biochemical identification/16S rDNA分类
农业科技引用本文复制引用
陈绍品,周碧君,程振涛,温贵兰,张升波,伍昌华,林汉卿,范昌龙,管国丹,汪德生,文明..贵州某鸡场大肠杆菌与葡萄球菌混合感染病原的分离鉴定[J].中国畜牧兽医,2017,44(5):1468-1476,9.基金项目
贵州大学博士基金(贵大人基合字201312号) （贵大人基合字201312号）
贵州省科技计划课题联合基金(黔科合LH字20147666号) （黔科合LH字20147666号）
贵州大学"本科教学工程"项目(JKSP2013004) （JKSP2013004）
贵州省科学技术基金(黔科合J字20152046号) （黔科合J字20152046号）
贵州省科技创新人才团队项目(黔科合人才团队20154016) （黔科合人才团队20154016）
