中国畜牧兽医2017，Vol.44Issue(5)：1477-1483,7.DOI:10.16431/j.cnki.1671-7236.2017.05.031
2株猪繁殖与呼吸综合征病毒的致病性分析
Pathogenic Analysis of Two Strains of Porcine Reproductive and Respiratory Syndrome Virus
摘要
Abstract
Samples of sick pigs suspected of porcine reproductive and respiratory syndrome (PRRS)infection were collected from Hebei and Fujian provinces in China in 2016.Studies were carried out to investigate the variation characteristics of the isolated virus strains.The virus iso-lates were propagated in Marc-145 cells and caused syncytial cytopathic effect.RT-PCR and indi-rect fluorescent assay (IFA)results showed that the isolates were HP-PRRSV and hence desig-nated as PRRSV CZ16A and NP16A,respectively.In order to study the pathogenicity of CZ16A and NP16A,the isolated strains were purified by plaque-isolation,and then inoculated to 60-days-old healthy pigs,which were negative in both of PRRSV infection and antibody.Clinical ob-servation,histopathological changes and viremia detection were carried out to evaluate the patho-genicity of these two strains of PRRSV.Results showed that both isolates could replicate in pigs and cause a high body temperature,but these two strains of PRRSV isolates could not cause mor-bidity or mortality in experimental animals.Autopsy results also showed that NP16A could cause consolidation of the lung,lymph node enlargement in tested animals,suggesting that its virulence was a little stronger than CZ16A.关键词
猪繁殖与呼吸综合征病毒/分离鉴定/致病性分析Key words
porcine reproductive and respiratory syndrome virus (PRRSV)/isolation and identifi-cation/pathogenic analysis分类
农业科技引用本文复制引用
李志,隋修锟,吴竞,鑫婷,李明,高新桃,姜一曈,侯绍华..2株猪繁殖与呼吸综合征病毒的致病性分析[J].中国畜牧兽医,2017,44(5):1477-1483,7.基金项目
中国农业科学院科技创新工程(ASTIP-IAS-11) （ASTIP-IAS-11）
