中国畜牧兽医2017，Vol.44Issue(5)：1526-1532,7.DOI:10.16431/j.cnki.1671-7236.2017.05.037
鸡血藤总黄酮对猪圆环病毒2型感染小鼠脾脏氧化应激的影响
Effect of Total Flavonoids of Spatholobus suberectus Dunn on Oxidative Stress in Mice Spleen Induced by PCV2
摘要
Abstract
The aim of this study was to investigate the effect of total flavonoids of Spatholobus su-berectus Dunn (TFSD)on porcine circovirus type 2 (PCV2 )induced oxidative stress in mice spleen.70 Kunming mice were divided into 7 groups:Control group,TFSD group (100 mg/(kg· BW)),PCV2 group,PCV2 + vitamin C (VC)group,and PCV2 + various concentrations of TFSD groups (25,50 and 100 mg/(kg·BW)).Mice were continuously treated with PCV2 via both intragastric administration and intraperitoneal injection for 3 d to establish oxidative stress models.From the 4th to 6th day,mice were intragastric administrated with saline,VC or TFSD, respectively,according to the grouping method.At the 7th day,the activities of xanthine oxidase (XOD),myeloperoxidase (MPO)and superoxide dismutase (SOD),the levels of glutathione (GSH)and oxidized glutathione (GSSG),and the ratio of GSH to GSSG in the mice spleen were analyzed.The results showed that PCV2 infection significantly upregulated the XOD and MPO activities and GSSG content(P <0.05),and dramatically downregulated the SOD activity,GSH level and the ratio of GSH to GSSG (P <0.05)in the mice spleen.Compared to PCV2 group,the SOD activity,GSH content and the ratio of GSH to GSSG in mice treated with TFSD were signif-icantly increased (P <0.05),while the activities of XOD and MPO and the level of GSSG were significantly decreased (P <0.05),showing better performance in the inhibition of PCV2 induced changes of oxidative stress associated enzyme activities and moledule levels than VC.In conclu-sion,TFSD had regulative effect on the oxidative stress induced by PCV2 in mouse spleen.关键词
鸡血藤总黄酮/猪圆环病毒2型(PCV2)/氧化应激/脾脏Key words
total flavonoids of Spatholobus suberectus Dunn/porcine circovirus virus type 2 (PCV2)/oxidative stress/spleen分类
医药卫生引用本文复制引用
付远妨,赵尉丹,杨潇,林霜,胡庭俊,陈海兰..鸡血藤总黄酮对猪圆环病毒2型感染小鼠脾脏氧化应激的影响[J].中国畜牧兽医,2017,44(5):1526-1532,7.基金项目
广西自然科学基金(2014GXNSFCA118019) （2014GXNSFCA118019）
广西教育厅科研项目(KY2015ZD007) （KY2015ZD007）
"大学生创新创业训练计划"创新训练项目(201610593081) （201610593081）
