中国畜牧杂志2017，Vol.53Issue(9)：26-32,68,8.DOI:10.19556/j.0258-7033.2017-09-026
基于RNA-Seq转录组测序技术揭示南方黄牛肉质嫩度调控相关的分子机制
Molecular Mechanism Analysis of Meat Tenderness in Chinese South Cattle Based on RNA-Seq Transcriptome Sequencing Technology
摘要
Abstract
To reveal the molecular mechanism of meat quality and excavate function genes related to tenderness in Chinese south cattle,this study selected Yunling,Wenshan and Simmental cattle at approximately 12 months of age as experimental object.Firstly,after slaughter,muscle tenderness of above cattle was measured at different stages of acid discharge (0,1,2,3,5,7d),which aimed at revealing the differences of meat quality among three varieties.Using transcriptome sequencing and bioinformatics analysis,we screened out differential expression genes related to meat quality,then real-time fluorescence quantification PCR was further performed.Results showed,meat tenderness of three varieties (Yunling,Wenshan and Simmental) showed a decreasing trend at different stages of acid discharge.At the same stage of acid discharge,the value of shear force showed the highest in Simmental,followed by Wenshan and Yunling,which indicated that the tenderness of Yunling was relatively good and Simmental was relatively poor.After cDNA library sequencing of longissimus dorsi between Yunling and Simmental,we screened out 3198 differential expression genes,of which 1750 genes were up-regulated in Yunling.Additionally,we found two important pathways related to meat quality,such as adipocytokine signaling pathway and protein processing in endoplasmic reticulum.Further qPCR verification and correlation analysis between gene expression level and shear force value,we ultimately identified Leptin and CAPN1 genes related to meat tenderness.This study screened and identified important pathways and key genes regulating meat tenderness,which will provide theoretical basis for genetic improvement of meat quality in Chinese south cattle.关键词
南方黄牛/嫩度/转录组Key words
Chinese south cattle/Tenderness/Transcriptome分类
农业科技引用本文复制引用
孟祥忍,张成龙,樊永亮,王文强,毛永江,黄必志,亏开兴,杨章平..基于RNA-Seq转录组测序技术揭示南方黄牛肉质嫩度调控相关的分子机制[J].中国畜牧杂志,2017,53(9):26-32,68,8.基金项目
863国家高技术研究发展计划项目(2013AA102505-5) （2013AA102505-5）
