中国畜牧杂志2017，Vol.53Issue(9)：33-37,5.DOI:10.19556/j.0258-7033.2017-09-033
盐津乌骨鸡ADFP、FATP1和ApoB基因表达量变化及其与脂肪性状的相关分析
Expression of ADFP, FATP1, ApoB and Correlation Analysis of Relative Quantity and Fat Deposition in Yanjin Black-bone Chicken
摘要
Abstract
In order to discover the influence that adipose differentiation-related protein gene (ADFP),fatty acid transport protein 1 (FATP1) and apolipoprotein B (ApoB) acted on fat traits of Yanjin Black-bone chickens,we adopted the SRBR Green I Real-Time PCR method to test the relative quantity ofADFP,FATP1 and ApoB gene in subcutaneous fat,abdominal fat,liver and chest muscle of Yanjin Black-bone chickens at 5 growth points,including 28 d,49 d,70 d,91 d and 112 d.The results showed that the expression of the ADFP gene in 28 d is for chest muscle ＞ abdominal fat ＞ subcutaneous fat ＞ liver,and the expression of chest muscle is significantly higher than the expression of the other three tissues (P ＜ 0.05),and there was not significant in the other four time points (P＞0.05).The expression of the FATP1 genes of all the tissues in 49 d show that chest muscle ＞ abdominal fat ＞ subcutaneous fat ＞ liver,and the expression of the chest muscle is significantly higher than the expression of the subcutaneous fat (P ＜ 0.05),and there was not significant in the other four time points (P＞0.05).The differrence of the expression of the ApoB gene was not significant between the tissues in the 5 time points,and the expression of the ApoB gene in liver is significantly higher than the expression of the other tissues.The change of the three genes in the four tissues had no significant correlation with the traits include live weight,subcutaneous fat thickness,comb weight (P ＞ 0.05).The change of the quantity expression of the three genes may play a role of regulation in the process of fat deposits.关键词
ADFP/FATP1/ApoB/脂肪Key words
ADFP/FATP1/ApoB/Fat分类
农业科技引用本文复制引用
相德才,赵智勇,张斌,饶军,覃兴合,李晶..盐津乌骨鸡ADFP、FATP1和ApoB基因表达量变化及其与脂肪性状的相关分析[J].中国畜牧杂志,2017,53(9):33-37,5.基金项目
云南省农业厅“云岭优质肉鸡配套系选育与示范推广”项目 （）
国家自然科学基金(31200924) （31200924）
云南省应用基础研究计划(2010ZC162) （2010ZC162）
昆明大学科学研究基金(YJL11003) （YJL11003）
