浙江农林大学学报2017，Vol.34Issue(5)：856-863,8.DOI:10.11833/j.issn.2095-0756.2017.05.012
马尾松谷胱甘肽过氧化物酶PmGPX基因的克隆及表达分析
Cloning and expression analysis of the PmGPX gene encoding glutathione peroxidase in Pinus massoniana
摘要
Abstract
The study PmGPX genes was played a role in Pinus massoniana insect resistance, that the great sig-nificance for in-depth insect-resistant resistance mechanism in Pinus massoniana. PmGPX, a cDNA region en-coding glutathione peroxidase, was cloned in the transcriptome sequencing analysis of one insect-resistant ma-terial in Pinus massoniana. Analyses included an amino acid sequence and an expression (qRT-PCR) analysis. Results showed that the total length of open reading frame (ORF) was 741 bp encoding 246 amino acids. The amino acid sequence analysis showed three high conservative character fields of the GPX gene family for this gene. The isoelectric point of PmGPX was PI 8.29, and the protein molecular weight was 27.08 kDa. In genetic clustering, compared with some other plants, PmGPX showed a higher homology with Picea sitchensis (91%) but a low homology with other monocots and dicotyledonous plants (64%-80%). However, homologies of the three typical conservative GPX family domains (G1, G2, G3) reached a higher level with other plants (82%-100%). The expression analysis indicated that PmGPX was expressed in all kinds of tissues with higher ex-pression levels in leaves (P<0.01), especially in young leaves (33.45 times more than the expression level in roots). Daily expression patterns were same in different insect-resistant materials and showed up-down-up-regu-lated trend the day. In insect-resistant material, expression of PmGPX reached a higher point earlier than that of control (P<0.05 in old leaves and tender stem). The expression analysis also indicated that PmGPX was involved in the insect-resistant defense system of P. massoniana. This study laid a foundation for further analy-sis of the PmGPX biological function and its role in insect-resistant defense with Pinus massoniana. [Ch, 5 fig. 29 ref.]关键词
林木育种学/马尾松/谷胱甘肽过氧化物酶/PmGPX基因/克隆与表达Key words
forest tree breeding/Pinus massoniana/glutathione peroxidase/PmGPX/cloning and expression analysis分类
农业科技引用本文复制引用
陈虎,贾婕,罗群风,吴东山,杨章旗..马尾松谷胱甘肽过氧化物酶PmGPX基因的克隆及表达分析[J].浙江农林大学学报,2017,34(5):856-863,8.基金项目
国家自然科学基金资助项目(31660219) （31660219）
广西 "八桂学者"专项经费资助项目(2011A015) （2011A015）
广西自然科学基金资助项目(2014GXNSFBA118104) （2014GXNSFBA118104）
