浙江农林大学学报2017，Vol.34Issue(5)：955-962,8.DOI:10.11833/j.issn.2095-0756.2017.05.025
山木通扦插繁殖及生根机制
Cutting propagation technology and rooting of Clematis finetiana
摘要
Abstract
To study cutting propagation techniques with one-year-old cuttings of C lematis finetiana and to in-vestigate the dynamic changes in activity of related oxidative enzymes [peroxidase (POD), indoleacetic acid oxidase (IAAO), and polyphenol oxidase (PPO)] as well as the content of soluble sugar, soluble protein, and starch, an L9 (34) orthogonal experiment was conducted with four treatments of exogenous hormones, hormone concentration, soaking time, and cutting matrix. Results indicated that rooting of C. finetiana was derived from the cortex. The effects of different exogenous hormones, hormone concentration, soaking time, and cutting ma-trix on rooting were notable by variance analysis. The best rooting formula was as follows:a matrix of river sand processed with Indole-3-butyric acid (1H-Indole-3-butanoic acid (IBA) 100 mg·L-1 for 2 h producing a rooting rate of 22.7%. In the rooting process, the content of soluble sugar increased during the induction and root growth periods, but decreased during the expression period and the late growth stage. Soluble protein content decreased gradually after cutting reaching a low point when most cuttings rooted. Starch content declined throughout the process. Also, the POD, IAAO, and PPO activities all increased first and then decreased reach-ing a peak in the early stages of expression. Additionally, greater nutrient content for the test group was found than the control, and peak oxidase activities of test groups arrived 10 d earlier than the control. This demon-strated that exogenous hormones could improve the nutrient utilization and oxidase activities of cuttings. [Ch, 3 fig. 4 tab. 20 ref.]关键词
园艺学/山木通/扦插繁殖/营养物质/氧化酶Key words
horticulture/Clematis finetiana/cutting propagation/nutrients/oxidases分类
农业科技引用本文复制引用
赵爽,刘志高,冯彬,季梦成..山木通扦插繁殖及生根机制[J].浙江农林大学学报,2017,34(5):955-962,8.基金项目
浙江省农业(林木)新品种选育重大科技专项(2016C02056-13-4) （林木）
