北京中医药大学学报2017，Vol.40Issue(7)：550-557,8.DOI:10.3969/j.issn.1006-2157.2017.07.005
补阳还五汤防治特发性肺纤维化的作用及机制探讨
Effect and mechanism of Buyang Huanwu Tang in prevention and treatment of idiopathic pulmonary fibrosis
摘要
Abstract
Objective To investigate the pharmacodynamic mechanism of Buyang Huanwu Tang (Yang-Tonifying Five-Returning Decoction) in treatment of bleomycin-induced idiopathic pulmonary fibrosis (IPF) in rats.Methods Male SD rats (n =144) were randomly divided into blank group,bleomycin group,prednisone group (0.004 2 g/kg) and high-dose Buyang Huanwu Tang group (27.32 g/kg,high-dose group),mid-dose Buyang Huanwu Tang group (13.66 g/kg,mid-dose group) and low-dose Buyang Huanwu Tang group (6.83 g/kg,low-dose group,each n =24).The model of IPF was induced by one-time intratracheal injection of bleomycin in all groups except of blank group.All groups were given corresponding medicinal after modeling for 24 h,and 8 rats were chosen from each group on the 7th d,14th d and 28th d respectively for collecting bronchoalveolar lavage fluid (BLAF) and lung tissue.The severity of pulmonary alveolitis was observed by using HE staining,and levels of tumor necrosis factor-α (TNF-α),interleukin-1β (IL-1β) and high mobility group box 1 (HMGB1) in BLAF were detected by using enzyme-linked immunosorbent assay (ELISA).Results Compared with blank group,fibrosis focal increased in alveolar septa,lung parenchyma structure was damaged,and levels of TNF-α,IL-1β and HMGB1 increased in bleomycin group at different time points (P ＜ 0.05).Compared with bleomycingroup,pulmonary alveolitis was relieved in varying degrees,and levels of TNF-α,IL-1β and HMGB1 decreased in high-dose group,mid-dose group and low-dose group.Conclusion Buyang Huanwu Tang can effectively reduce the levels of IL-1 β,TNF-α and HMGB1 in BLAF,relieve alveolitis and inhibit pulmonary fibrosis in rats with bleomycin-induced IPF.关键词
补阳还五汤/肺纤维化/博莱霉素/炎性因子/大鼠Key words
Buyang Huanwu Tang (Yang-Tonifying Five-Returning Decoction)/pulmonary fibrosis/bleomycin/inflammatory factors分类
医药卫生引用本文复制引用
杨昆,李勇华,王飞,董滟..补阳还五汤防治特发性肺纤维化的作用及机制探讨[J].北京中医药大学学报,2017,40(7):550-557,8.基金项目
国家自然科学基金资助项目(No.81373597)National Natural Science Foundation of China (No.81373597) （No.81373597）
