北京中医药大学学报2017，Vol.40Issue(7)：564-571,8.DOI:10.3969/j.issn.1006-2157.2017.07.007
基于促BMSCs成骨诱导分化作用的鹿茸药材品质评价研究
Review on quality of Lurong (cornu cervi Pantotrichum) based on osteoinductive differentiatione effect of bone mesenchymal stem cells
摘要
Abstract
Objective To investigate the influences of medicated serum of Lurong (cornu cervi Pantotrichum) in various specifications on proliferation and osteogenic differentiation of bone mesenchymal stem cells (BMSCs),and conduct a cluster analysis on Lurong in 8 specifications.Methods SD rats (n =72) were randomly divided into 9 groups.The medical materials of Lurong in 8 specifications were screened according to the growth characteristics and processing methods of antlers.The medicated serum samples contained Lurong in various specifications were prepared respectively in rats,and BMSCs were cultured in these serum samples.The proliferation of BMSCs was detected by using methyl thiazolyl tetrazolium test (MTT),and secretion volume of alkaline phosphatase (ALP),bone morphogenetic protein-2 (BMP-2) and bone gla-protein (BGP) were detected by using enzymelinked immunosorbent assay (ELISA).The test results were statistically analyzed by applying SPSS17.0 software and grey relational analysis respectively.Results The medicated serum of Lurong had an accelerative effect on proliferation of BMSCs,which was superior in Lurong wax-scale groups to that in inducing liquid group.The serum also improved the secretion of ALP,BGP and BMP-2 during the course of osteogenic differentiation of BMSCs.The ranking of Lurong in different qualities,according to the effects of tonifying the kidney and invigorating bone,showed that the results of quality reviews on samples of Lurong in 8 specifications conformed to the traditional quality partition of Lurong medical materials.Conclusion This method and model can be applied in quality review of medical materials of Lurong.关键词
鹿茸/品质评价/骨髓间充质干细胞/灰关联度分析/大鼠Key words
Lurong (cornu cervi Pantotrichum)/quality review/bone mesenchymal stem cells/grey relational analysis/rats分类
医药卫生引用本文复制引用
曲雷鸣,李峰,龚伟,刘威..基于促BMSCs成骨诱导分化作用的鹿茸药材品质评价研究[J].北京中医药大学学报,2017,40(7):564-571,8.基金项目
国家自然科学基金面上资助项目(No.81473314),辽宁省自然科学基金资助项目(No.201705406)National Natural Science Foundation of China (No.81473314),Liaoning Natural Science Foundation (No.201705406) （No.81473314）
