北京中医药大学学报2017，Vol.40Issue(7)：572-577,6.DOI:10.3969/j.issn.1006-2157.2017.07.008
缬草醛对肠易激综合征模型大鼠胃肠敏感性和结肠中5-HIAA及5-HT相关受体蛋白表达的影响作用
Influences of baldrinal on gastrointestinal sensitivity and expressions of 5-HIAA and relevant receptors of 5-HT in rats with irritable bowel syndrome
摘要
Abstract
Objective To investigate the effect of baldrinal on irritable bowel syndrome (IBS) induced by chronic-acute stress and its influences on the protein expressions of 5-hydroxyindoleacetic acid (5-HIAA) and relevant receptors of 5-hydroxytryptamine (5-HT) in rats.Methods SD rats were randomly divided into control group,model group,high-dose baldrinal group (0.6 mg/kg),mid-dose baldrinal group (0.3 mg/kg),low-dose baldrinal group (0.15 mg/kg) and pinaverium group (25 mg/kg).The rat model of IBS was established by using chronic-acute stress combined with independent feeding for observing the influence of baldrinal on the scores of abdominal withdraw reflection (AWR).The content of colonic 5-HIAA was detected by using high performance liquid chromatography (HPLC),and influences of baldrinal on protein expressions of colonic 5-HT1 A,5-HT3A and 5-HTR4 were detected by using Western blotting.Results Thc scores of AWR content of colonic 5-HIAA were decreased in high-dose baldrinal group,middose baldrinal group and low-dose baldrinal group compared with model group (P ＜ 0.05).The protein expressions of colonic 5-HT1A,5-HT3A and 5-HTR4 were regulated in high-dose baldrinal group,middose baldrinal group and low-dose baldrinal group.Conclusion Baldrinal has significant inhibitory effect on gastrointestinal hyperfunction in rat model of IBS and reduce viscera sensitivity,which indicates that baldrinal can relieve IBS symptoms.The effective mechanism may be related to the regulation effect of baldrinal on 5-HIAA contents and protein expressions of relevant receptors of 5-HT.关键词
缬草醛/肠易激综合征/5-羟吲哚乙酸/5-HT受体/大鼠Key words
baldrinal/irritable bowel syndrome/5-hydroxyindoleacetic acid/receptors of 5-hydroxytryptamine分类
医药卫生引用本文复制引用
陶丝雨,肖丛瑞,王晶,张淑静,高誉珊,穆芳园,王一程,闫兴丽..缬草醛对肠易激综合征模型大鼠胃肠敏感性和结肠中5-HIAA及5-HT相关受体蛋白表达的影响作用[J].北京中医药大学学报,2017,40(7):572-577,6.基金项目
国家自然科学基金资助项目(No.81374044),北京中医药大学校级自主课题资助项目(No.2016-JYB-XS028)National Natural Science Foundation of China (No.81374044) （No.81374044）
