北京中医药大学学报2017，Vol.40Issue(7)：578-584,7.DOI:10.3969/j.issn.1006-2157.2017.07.009
5种有“味酸”药性的蔷薇科果实类药材中水溶性有机酸的测定与分析
Water-soluble organic acids in 5 fruit medicinal with sour taste from rosaceae family
摘要
Abstract
Objective To probe the similarities and differences in water-soluble organic acids among 5 fruit medicinals with sour taste from rosaceae family [Shanzha (Crataegi Fructus),Mugua (Chaenomelis Fructus),Wumei (Mume Fructus),Jinyingzi (Rosae Laevigatae Fructus) and Fupenzi (Rubi Fructus)]through detecting their pH value and organic acid content.Methods The pH value and content of total titratable organic acids in 5 fruit medicinals were determined by using direct potentiometric method and potentiometric titration.The content of water-soluble organic acids including quinic acid,malic acid,shikimic acid,citric acid,fumaric acid,protocatechuic acid and chlorogenic acid were determined by using high performance liquid chromatography (HPLC) and three-wavelength switching method.Results The pH value of 5 fruit medicinals was in an ascending order as follows:Wumei (2.64) ＜ Shanzha (3.19) ＜ Mugua (3.28) ＜ Fupenzi (4.33) ＜ Jinyingzi (5.06).The content of total titratable organic acids was in a descending order as follows:Wumei (16.2％) ＞ Shanzha (8.39％) ＞ Mugua (7.19％)＞ Jinyingzi (3.97％) ＞ Fupenzi (3.58％).Mugua and Wumei contained nearly all of detected 7 organic acids,and chlorogenic acid was not detected from Jinyingzi and chlorogenic acid and quinic acid were not detected from Fupenzi.The organic acids with higher content in 5 fruit medicinals were,respectively,citric acid (1.09％ in Wumei,0.290％ in Shanzha and 0.272％ in Fupenzi),shikimic acid (0.753％ in Mugua) and quinic acid (0.277％ in Jinyingzi).The results of data analysis indicated that pH value and content of total titratable organic acid showed a good negative correlation (Pearson correlation coefficient was-0.73,P ＜ 0.01).The content changes of total titratable organic acids and citric acid showed nearly equal in 5 fruit medicinal.The comparison in content of each organic acid showed that the changes of spectrums of monomeric organic acid were different among 5 fruit medicinal.Conclusion This experiment conducts a fairly comprehensive analysis on water-soluble organic acids in 5 fruit medicinal,provides thinking and accumulates experimental data for the systematical characterization and quantification of important content in Chinese medicinal with sour taste.Meanwhile the experiment provides the evidence for Pharmacopoeia of People's Republic of China that citric acid can be taken as the quality marker for Wumei and Shanzha.关键词
蔷薇科/果实类药材/水溶性有机酸/电位法/高效液相色谱法Key words
rosaceae/fruit medicinal/water-soluble organic acids/potentiometric method/high performance liquid chromatography分类
医药卫生引用本文复制引用
曹富,况作品,谢晓梅,吴蓓丽,张玲..5种有“味酸”药性的蔷薇科果实类药材中水溶性有机酸的测定与分析[J].北京中医药大学学报,2017,40(7):578-584,7.基金项目
“十二五”国家科技支撑计划资助项目(No.2011BAI04B06),安徽省高校自然科学研究重点资助项目(No.KJ2015A128),安徽中医药大学探索性科研资助项目(No.2016ts074)National Sci-Tech Support Plan of the 12th Five-Year Plan (No.2011BAI04B06),Natural Science Research of Universities in Anhui Province (No.KJ2015A128) （No.2011BAI04B06）
