长春中医药大学学报2017，Vol.33Issue(5)：696-699,4.DOI:10.13463/j.cnki.cczyy.2017.05.003
三黄胶囊对急性痛风性关节炎的抗炎作用
Experimental research of Sanhuang capsule on acute gouty arthritis
摘要
Abstract
Objective To observe the effects of Sanhuang capsule oninter IL-β, TNF-α and COX-2 levels in the serum and the ankle joint fluid, and explore the therapeutic mechanism of Sanhuang capsule on gouty arthritis induced by MSU crystal in rats. Methods The rats were divided into normal group, model group, Sanhuang capsule group (6.45g/kg), colchicine group (0.325 mg/kg). The normal group and model group were ig (intragastric administration) administered with normal saline, various treatment groups were ig administered with corresponding doses for continuous 14 days. One hour after the last administering, 100 l sterile 1% PBS was injected in on the right side ankle joint cavity in normal group, and 100l MSU was injected in other groups. Twenty-four hour after modeling, ELISA method were used to detect IL-, TNF-α or COX-2 levels in the serum and ankle joint fluid. and the pathologic changes of synovial tissues were observed by microscope. Results Compared with the normal group, the levels of IL-and TNF-α in serum and IL-β,TNF-α,COX-2 in ankle joint fluid were significantly increased after injection of MSU into the ankle joint of rats (P<0.05) after 24h. Compared with the model group, the levels of IL-β and TNF-α in the rat and IL-β, TNF-α, COX-2 in ankle joint fluid induced by MSU were significantly decreased in Sanhuang Capsule and colchicine group (P<0.05). the total index of synovial tissue of model of inflammatory cell infiltration, fiber optic proliferation, synovail cell proliferation, edema, congestion were more severe than those in the Chinese herbs group and western Medicine group. Conclusion MSU can increase the levels of IL-β,TNF-α in serum and IL-β, TNF-α and COX-2 in ankle joint fluid. Sanhuang capsule may have the effect on MSU induced gouty arthritis by decreasing IL-β, TNF-α and COX-2 level.关键词
三黄胶囊/尿酸钠/急性痛风性关节炎Key words
Sanhuang capsule/monosodium urate/acute gouty arthritis分类
医药卫生引用本文复制引用
李梅珍,唐秋月,梁国强..三黄胶囊对急性痛风性关节炎的抗炎作用[J].长春中医药大学学报,2017,33(5):696-699,4.基金项目
江苏省青年医学人才培养计划项目(QNRC2016252) （QNRC2016252）
江苏省苏州市科技局计划项目(SYS201420). （SYS201420）
