重庆医学2017，Vol.46Issue(26)：3605-3607,3610,4.DOI:10.3969/j.issn.1671-8348.2017.26.002
色素上皮衍生因子对缺氧条件下H9C2心肌细胞保护作用
Protective effect of PEDF on H9C2 myocardial cells under hypoxia condition
摘要
Abstract
Objective To investigate the protective effects and possible mechanism of pigment epithelium derived faetor (PEDF) on myocardial cells H9C2 under hypoxia and serum-free condition.Methods H9C2 cells were culture in vitro and performed the hypoxia and serum-free processing.The cells were divided into the control group (H9C2),hypoxia group (hypoxia + H9C2),PEDF group(hypoxia+H9C2 +PEDF) and mitochondrial fission inhibitor(Mdivi-1) group(hypoxia+h9C2+Mivi-1).The apoptotic rate was detected by TUNNEL staining.The proteins levels of dynamin related peptide1 (Drp1) and cleaved-caspase 3 were measured by Western blot.Electron Microscopy and MitoTracker Red were used to detect the mitochondria morphology,the mitochondrial membrane potential was evaluated by cationic dye JC-1.MitoSOXTM was used to detect mitochondrial reactive oxygen species (ROS).Results Hypoxia induced mitochondrial fission(P＜0.05).The hypoxia group (6 h) and control group had statistical difference(P＜0.05).PEDF reduces mitochondrial fission under hypoxia condition(P＜0.05),which had statistical difference between the PEDF group and hypoxia group (6 h)(P＜0.05).PEDF and Mdivi-1 could decrease cell apoptosis under hypoxia condition(24 h),compared with the hypoxia group,the difference was statistically significant(P＜0.05).Conclusion PEDF decrease cell apoptosis by inhibiting H9C2 cells mitochondrial fission under hypoxia condition.关键词
细胞凋亡/缺氧/心脏/H9C2细胞/色素上皮衍生因子/动力相关蛋白1/线粒体分裂Key words
apoptosis/anoxia/cardiac/H9C2 cells/pigment epithelium derived factor/dynamin related peptide1/mitochondrial fission分类
医药卫生引用本文复制引用
王柱,周中新,关秋华,张昊,刘志伟,董红燕,张中明..色素上皮衍生因子对缺氧条件下H9C2心肌细胞保护作用[J].重庆医学,2017,46(26):3605-3607,3610,4.基金项目
国家自然科学基金资助项目(81270173) （81270173）
江苏省卫生厅科技计划项目青年基金(Q201407). （Q201407）
