重庆医学2017，Vol.46Issue(26)：3614-3616,3.DOI:10.3969/j.issn.1671-8348.2017.26.005
血红素加氧酶-1在高氧肺损伤小鼠中的表达及作用
Expression and role of heme oxygenase-1 in mouse lung injury induced by hyperoxia
摘要
Abstract
Objective To investigate the expression of heme oxygenase-1 in lungs of transgenic mice and normal mice and its effects.Methods 32 newborn mice were divided into four groups:wild-type group (WT group),specific transgenic mice expressed hemoglobin oxygenase-1 high level group[HO-1-FL(H) group,cytoplasm],low level WT group[HO-1-FL(L) group,cytoplasm] and the C-terminal heme oxygenase-1 group(Nuc-HO-1-TR group,nucleus).Then four groups of mice were exposed to high oxygen for 3 days,after that placed in normal air environment.Immunohistochemistry and immunofluorescence were used to observe the alveolar development and hemoglobin addition at 3 days,7 days and 14 days oxygenase-1 expression in the lungs of mice.Results The levels of heme oxygenase-1 were significantly higher in the lungs of HO-1-FL (H) group,and the alveolar development in the lungs of the four groups after 3 days of hyperoxia exposure was impaired.In the normal air environment,the recovery of alveolar development in HO-1-FL (H) group on the 7th and 14th day was the best.Conclusion The moderately high levels of heme oxygenase-1 expression in the lungs contribute to the recovery of lung injury in mice caused by hyperoxic environments.关键词
肺损伤/血红素加氧酶-1/基因敲除小鼠Key words
pulmonary injury/heme oxygenase-1/knockout mice分类
医药卫生引用本文复制引用
罗喜钢,王毅,张凤香..血红素加氧酶-1在高氧肺损伤小鼠中的表达及作用[J].重庆医学,2017,46(26):3614-3616,3.基金项目
国家自然科学基金资助项目(81541099). （81541099）
