重庆医学2017，Vol.46Issue(26)：3617-3619,3.DOI:10.3969/j.issn.1671-8348.2017.26.006
1,25(OH)2D3对溃疡性肠炎小鼠红细胞及脾脏免疫功能的影响
Effects of 1,25-dihydroxy vitamin D3 on immune function of RBC and spleen in ulcerative colitis mice
赵红伟 1张建 1和丽丽 2郎晓猛 3朱叶珊 4刘建平3
作者信息
- 1. 河北省人民医院消化内科,石家庄050051
- 2. 河北省人民医院老年病科,石家庄050051
- 3. 河北省中医院脾胃科,石家庄050071
- 4. 河北省唐山市中医院脾胃科 063000
- 折叠
摘要
Abstract
Objective To investigate the effects of 1,25-dihydroxy vitamin D3 [1,25 (OH)2D3] on immune mechanism of RBC and spleen in colitis model mice.Methods Thirty mice were randomly grouped as follows:blank control group,model group and 1,25(OH)2D3 group.The animal models were established.Then the general condition and colon pathological changes in mice were observed,the changes of the RBC immune compound(RCIC) and RBC surface C3b receptor(RC3bR) wreath were detected by the yeast wreath method,the weight and length of spleen were measured,the positive rates of spleen immune cells were detected by flow cytometry.Results Compared with the model group,RBC-C3bR in the 1,25(OH)2D3 group and blank control group was significantly increased and RBC-ICR was significantly decreased (P＜0.01).Compared with the blank control group,the positive rates of CD3,CD4,CD8 and CD45R in spleen mononuclear cell were significantly increased (P＜0.01);after the 1,25 (OH) 2D3 intervention,the positive rates of CD3,CD4,CD8 and CD56R in spleen mononuclear cells were significantly decreased compared with the model group(P＜0.01).Conclusion 1,25(OH)2D3 has the immune regulatory effect on RBC and peripheral spleen lymphocytes in chronic colitis mice.关键词
1,25(OH)2D3/红细胞免疫/脾脏淋巴细胞/溃疡性结肠炎/慢性动物模型Key words
1,25 (OH) 2D3/RBC immune/spleen lymphocytes/ulcerative colitis/chronic animal model分类
医药卫生引用本文复制引用
赵红伟,张建,和丽丽,郎晓猛,朱叶珊,刘建平..1,25(OH)2D3对溃疡性肠炎小鼠红细胞及脾脏免疫功能的影响[J].重庆医学,2017,46(26):3617-3619,3.
