重庆医学2017，Vol.46Issue(26)：3623-3625,3628,4.DOI:10.3969/j.issn.1671-8348.2017.26.008
TLS9a核酸适配体对小鼠肝癌细胞的靶向作用研究
Targeting effect of TLS9a nucleic acid aptamer on mice hepatic cancer cells
魏莉平 1王镀津 2邹甜甜 1费安兴1
作者信息
- 1. 湖北省黄石市妇幼保健院团城山院区检验科 435000
- 2. 湖北省黄石市中心医院检验科 435000
- 折叠
摘要
Abstract
Objective To investigate the targeting effect of TLS9a nucleic acid aptamer on mice hepatic cancer cells.Methods The liposome modified with maleimide and loading doxorubicin(DOX) was prepared,then TLS9a nucleic acid aptamer modified by FITC fluorescence and sulfydryl was synthesized,which was coupled to the liposome surface.The entrapment efficiency of DOX was detected by UV spectrophotometry.The dynamic light scattering(DLS) was applied to measure the particle size of nanoparticles and the potential distribution.The uptake of DOX in mice hepatic cancer cells was detected by the Nikon inverted microscope and the mean fluorescence intensity of liposome/DOX and TLS9a-liposome/DOX was detected by flow cytometry.The cells activity was detected by MTT.Results Flow cytometry assay showed that the binding rate of TLS9a nucleic acid aptamer with BNL.1ME.A.7R.1 mice hepatic cancer cells was 54.1％.TLS9a-liposome particle size distribution was in (116.0 ± 5.0)nm.TLS9a-liposome/DOX released DOX quickly at pH 5.0,and the release amount in 72 h was more than 70 ％ of the total release amount.TLS9a-liposome/DOX effectively inhibited the growth of mice hepatic cancer cells BNL.1ME.A.7R.1.Conclusion TLS9a nucleic acid aptamer could specifically combined with mice hepatic cancer cells BNL.1ME.A.7R.1,which could be used to detect mice hepatic cancer cells.关键词
肝肿瘤/核酸适配体/TLS9a/靶向治疗/小鼠肝癌细胞Key words
liver neoplasms/nucleic acid aptamer/TLS9a/targeted therapy/mice hepatic cancer cells分类
医药卫生引用本文复制引用
魏莉平,王镀津,邹甜甜,费安兴..TLS9a核酸适配体对小鼠肝癌细胞的靶向作用研究[J].重庆医学,2017,46(26):3623-3625,3628,4.
