重庆医学2017，Vol.46Issue(27)：3745-3747,3751,4.DOI:10.3969/j.issn.1671-8348.2017.27.001
甘草酸对高糖诱导的肾小球系膜细胞氧化应激损伤的影响
Influence of glycyrrhizic acid on oxidative stress injury of glomerular mesangial cells induced by high glucose
摘要
Abstract
Objective To investigate the influence of glycyrrhizic acid (GA) on oxidative stress injury in glomerular mesangial cells (HBZY-1) induced high glucose.Methods HBZY-1 was cultured and divided into the normal control group,high glucose group and high glucose +-GA group.The cell proliferation activity was measured by MTT assay.UV spectrophotometry was used to detect the SOD and MDA levels.And the ROS changes were detected by laser confocal microscopy.Immunohistochemistry and Western blot were used to detect the expression of Mn-SOD protein in various groups.Mn-SOD mRNA was detected by Q-PCR.Results (1) The morphological changes in each group:HBZY-1 cells were diamond-shaped,cells were normal and the structure was clear in the NG group.In the HG+-GA group,the number of cells was increased slightly,individual cell body was slightly hypertrophy.The cell structure in the HG group was not clear,the cells appeared flat,the number was increased significantly,the cell body was slightly hypertrophy.(2)The proliferative effect of HBZY-1 in each group:compared with the NG group,the OD value in the HG group was significantly increased,and the OD value in the HG+GA group was decreased compared with the HG group(P＜0.05).(3)The relative content of ROS in the HG group was higher than that in the NG group,and which in the HG+GA group was decreased (P＜0.05).(4)The expression of Mn-SOD in each group:the relative expression of Mn-SOD in the HG group was significantly lower than that in the NG group (P＜0.05),and the expression of Mn-SOD in the HG+-GA group was increased compared with the HG group(P＜0.05).Conclusion Glycyrrhizic acid has a certain inhibiting effect on the abnormal proliferation of HBZY-1 induced by high glucose.Glycyrrhizic acid can protect the cell hypertrophy and cell damage caused by glomerular mesangial cells via oxidative stress,and glycyrrhizic acid can regulate the Mn-SOD expression induced by high glucose.关键词
甘草酸/肾小球系膜细胞/氧化应激Key words
glycyrrhizic acid/mesangial cell/oxidative stress分类
医药卫生引用本文复制引用
侯绍章,张婷,李媛,伍智慧..甘草酸对高糖诱导的肾小球系膜细胞氧化应激损伤的影响[J].重庆医学,2017,46(27):3745-3747,3751,4.基金项目
国家自然科学基金资助项目(81160106). （81160106）
