重庆医学2017，Vol.46Issue(27)：3748-3751,4.DOI:10.3969/j.issn.1671-8348.2017.27.002
N-乙酰-L-色氨酸对大鼠肝脏缺血再灌注后肠损伤的保护作用
Protective effect of N-acetyl-L-tryptophan on intestinal damage after rat hepatic ischemia reperfusion
摘要
Abstract
Objective To investigate the protective effects of N-acetyl-L-tryptophan (L-NAT) on intestinal damage after rat hepatic ischemia reperfusion.Methods Twenty-four healthy adult rats were divided into the sham operation group (Sham),ischemia reperfusion group (IR),ischemia reperfusion and N-acetyl-L-tryptophan group(IR+L-NAT).The hepatic ischemia reperfusion model was established by occluding the afferent vessels of the left and middle lobes.The morphological structures of the small intestine were observed by hematoxylin-eosin (HE) staining.The expressions of active caspase-3,Bax and Bcl-2 were detected by immunohistochemistry staining.Results (1) In the IR group,the structure of intestinal villis was destroyed,the intestinal mucosa showed congestion and exfoliation,the epithelial cells had degeneration and necrosis,and infiltration of inflammatory cells appeared;which could be alleviated by L-NAT.(2)The immunohistochemistry showed that compared with the Sham group,the expression of active caspase-3,Bcl-2 and Bax in the IR group was increased,after L-NAT intervention,the Bax and caspase-3 expression was decreased,while the Bcl-2 expression was further increased.Conclusion L-NAT could inhibit the apoptosis of small intestinal epithelial cells caused by liver ischemic reperfusion and attenuates intestinal epithelial damage.关键词
缺血再灌注损伤/小肠/N-乙酰-L-色氨酸/细胞凋亡/大鼠Key words
ischemia-reperfusion injury/small intestine/N-acetyl-L-tryptophan/apoptosis/rat分类
医药卫生引用本文复制引用
王淑惠,姜红心,李晓双,胡梦琪,于海,张明,于树娜,王健欣,蒋吉英..N-乙酰-L-色氨酸对大鼠肝脏缺血再灌注后肠损伤的保护作用[J].重庆医学,2017,46(27):3748-3751,4.基金项目
山东省自然科学基金资助项目(ZR2010HM006 （）
ZR2014HL020 （）
ZR2014HL021) （）
山东省教育厅资助课题(J11LF14) （J11LF14）
山东省医药卫生科技发展计划资助课题(2014WS0464) （2014WS0464）
潍坊医学院科技创新研究基金资助项目(K1301001) （K1301001）
潍坊医学院大学生科技创新基金资助项目(KX20150091) （KX20150091）
2015年地方高校国家级大学生创新创业训练计划(201510438010 （）
201510438007). （）
