重庆医学2017，Vol.46Issue(27)：3752-3754,3758,4.DOI:10.3969/j.issn.1671-8348.2017.27.003
乙型肝炎病毒抑制肝细胞免疫检查点PD-1配体基因表达的研究
Hepatitis B virus inhibits expression of liver cell immune checkpoint PD-1 ligand gene
摘要
Abstract
Objective To analyze the impact of hepatitis B virus (HBV) replication and its coded X gene (HBx) expression on cell gene expression profile,especially the immune related gene expression level changes.Methods Overexpressed HBx gene was transiently transfected through lenti-virus and pcDNA3,the RNA-Seq and RT-qPCR methods were used to detect the expression levels of immune related genes,which were verified in HBV replication cell line.Results This study found that the HBV replication and HBx expression suppressed the expression of immune checkpoint PD-1 ligand gene (PD-L1/CD274) in a dose-dependent manner,while the expression of H-box mutant in HBx gene lost this inhibition effect.Conclusion HBV/HBx possesses the ability for inhibiting PD-L1/CD274 ligand gene expression,may relieve the checkpoint of antigen-specific T cell activation in viral infection acute stage,activates cytotoxic T cells,which may cause that T cell attack and clear highly replicated cells,helps virus to enter the lower replication status,and reaches the balance status between virus-host and lays a basis of HBV chronic infection.关键词
肝炎病毒,乙型/HBx/免疫检查点/PD-L1/CD274Key words
hepatitis B virus/HBx/immune checkpoint/PD-L1/CD274分类
医药卫生引用本文复制引用
郭莲娣,王丹,唐子执,曾鸣,王小军,刘聪,李友伟..乙型肝炎病毒抑制肝细胞免疫检查点PD-1配体基因表达的研究[J].重庆医学,2017,46(27):3752-3754,3758,4.基金项目
四川省科技厅科技支撑项目(2017FZ0034) （2017FZ0034）
四川省卫生和计划生育委员会基金资助项目(16PJ144) （16PJ144）
国家级大学生创新创业训练计划项目(201610656073). （201610656073）
