重庆医学2017，Vol.46Issue(27)：3763-3766,4.DOI:10.3969/j.issn.1671-8348.2017.27.006
银杏叶提取物对肠炎小鼠肠黏膜屏障的保护作用研究
Protective role of Gingko Biloba extract in intestinal mucosal barrier of intestinal inflammation mouse
摘要
Abstract
Objective To investigate the protective role of Gingko Biloba extract (EGB 761) in intestinal mucosal barrier of intestinal inflammation model mouse.Methods Thirty mice were randomly divided into the control group,model group and EGB 761 group;the animal model was established;the general condition,body mass change,fecal occult blood and colon histopatho1ogical changes were observed,the expressions of occludin-1 proteins in colon tissue were detected by immunofluorescence.Results The body mass in the model group appeared to decrease,drinking water and eating food were significantly decreased compared with that in the control group.However after the EGB 761 intervention,the body mass of the model mice was significantly risen again;little inflammatory cells infiltration could be seen in the EGB 761 group,the inflammatory degree was significantly alleviated compared with the model group.The fluorescence distribution of the claudin-1,occludin and zo-1 in colonic tissues of the model group was more disperse compared with the normal group,the fluorescence intensity was weakened with rough edge;after EGB 761 intervention,the fluorescence in the EGB7 61 group was distributed along with the cellular membrane,the intensity was slightly weakened compared with the normal control group,but was still stronger than that in the model group.Conclusion EGB 761 can improve the inflammatory reaction in mouse colonic tissue,its mechanism may be related with its strengthening the protective effect of intestinal mucosal barrier.关键词
银杏叶提取物/溃疡性结肠炎/急性期动物模型/肠黏膜屏障Key words
EGB 761/ulcerative colitis/acute stage animal model/intestinal mucosal barrier分类
医药卫生引用本文复制引用
赵红伟,和丽丽,韩华,岳月红,康薇,朱叶珊,刘建平..银杏叶提取物对肠炎小鼠肠黏膜屏障的保护作用研究[J].重庆医学,2017,46(27):3763-3766,4.基金项目
河北省中医药管理局科研计划项目(2016180). （2016180）
