医学分子生物学杂志2017，Vol.14Issue(5)：268-273,6.DOI:10.3870/j.issn.1672-8009.2017.05.000
乙酰左旋肉碱对人类精子冷冻前后顶体完整性及超微结构的保护作用探讨
Protective Role of Acetyl-L-Carnitine on Ultrastructure and Acrosome Integrity of Human Spermatozoa after Cryopreservation
摘要
Abstract
Objective To investigate role of acetyl-L-carnitine on ultrastructure and acrosome integrity of human spermatozoa after freezing-thawing process. Methods The spermatozoa were col-lected from 18 male patients and divided into two groups after centrifugation. The cryopreservation of human spermatozoa for two weeks treated with acetyl-L-carnitine at 7. 5 mmol/L ( group B ) was compared with control ( group A: no acetyl-L-carnitine given ) . For the frozen-thawed spermato-zoa, the viability, motility, ultrastructure and acrosome integrity by FITC-PNA staining was deter-mined in each group. Results Motility and viability of thawed spermatozoa was significantly lower than that of pre-frozen ( P<0. 05 ) , while the motility and viability of spermatozoa in group B was significantly increased as compared with those in group A ( P<0. 05 ) . Damaged proportion of head and tail ultrastructure of spermatozoa was significantly higher than that of pre-frozen ( P<0. 05 ) , while damaged proportion of head and tail ultrastructure of spermatozoa in group B was significantly lower as compared with those in group A ( P <0. 05 ) . Acrosome integrity of thawed spermatozoa was significantly lower than that of pre-frozen ( P<0. 05 ) , while acrosome integrity of spermatozoa in group B was significantly increased as compared with those in group A ( P<0. 05 ) . Conclusion Acetyl-L-carnitine at a concentration of 7. 5 mmol/L is an effective antioxidant against cryo-dam-age on ultrastructure and acrsome integrity of post-thawed human spermatozoa.关键词
乙酰左旋肉碱/冷冻损伤/超微结构/顶体完整性Key words
acetyl-L-carnitine/cryodamage/ultrastructure/acrosome integrity分类
医药卫生引用本文复制引用
邹宇洁,常硕,杨菁,尹太郎,穆杨,周琪..乙酰左旋肉碱对人类精子冷冻前后顶体完整性及超微结构的保护作用探讨[J].医学分子生物学杂志,2017,14(5):268-273,6.基金项目
资助项目:湖北省自然科学基金(No. 2016 CFB352),湖北省卫计委面上项目(No. WJ2017 MO11),中国国家留学基金(No. 201706275124) This work was supported by grants from the Natural Science Foundation of Hubei Province(No. 2016 CFB352),Hubei Province Health and Family Planning Scientific Research Project(No. WJ2017 MO11)and China Scholarship Council(No. 201706275124) （No. 2016 CFB352）
