河南中医2017，Vol.37Issue(9)：1566-1569,4.DOI:10.16367/j.issn.1003-5028.2017.09.0548
茵陈、大黄联合用药对四氯化碳肝损伤小鼠的保护作用
Protective Effects of Yinchen (Herba Artemisiae Scopariae) and Dahuang (Radix et Rhizoma Rhei) on Mice Liver Injury Induced by CCI
摘要
Abstract
Objective:To compare the protective effects of combination of Yinc hen(Herba Artemisiae Scopariae) and Dahuang (Radix et Rhizoma Rhei) (Yin Huang combination) of different proportions on mice liver injury induced by CCl and explore the optimal proportion of Yinchen (Herba Artemisiae Scopariae) and Dahuang (Radix et Rhizoma Rhei) for the mice liver injury induced by CCl.Methods:Totally 80 mice were randomized into blank group,model group,positive control group and Yin Huang (10 ∶ 0) group,Yin Huang (9 ∶ 1) group,Yin Huang (3 ∶ 1) group,Yin Huang (3 ∶ 2) group,Yin Huang (1 ∶ 1) group,Yin Huang (1 ∶ 9) group,Yin Huang (0 ∶ 10) group,8 mice in each group.Positive control group were performed gavage of Bifendate (0.2 g · kg-1) while treatment group were given gavage of Yin Huang combination of the above proportions.Blank group and model group were given gavage with distilled water of same volume.Each group was given continuous gastral administration of 7 days.One hour after the last administration,administration groups and model group were given single abdominal injection of carbon tetrachloride peanut oil solution (0.01 mL · g-1) with mass score of 0.1％.After 16h,the blood was taken by removing eyeballs,and the levels of ALT and AST were measured.The weight of liver was determined by taking out the liver and the hepatosomatic index was calculated.Liver tissue bomogenate was made and the concentrations of TG and TC were determined.Tissue sections were made bv the same part of the liver,and the pathologic morphology was observed after HE staining.Results:Compared with model group,Yin Huang combination with different proportions can reduce the hepatosomatic index in mice with liver injury;the difference had statistical significance (P ＜ 0.05).Combined with blank group,the levels of ALT and AST increased markedly;compared with model group,levels of ALT in Yin Huang (10 ∶ 0) group,Yin Huang (9 ∶ 1) group and Yin Huang (1 ∶ 9) group declined;levels of AST in Yin Huang (10 ∶ 0) group,Yin Huang (3 ∶ 2) group,Yin Huang (1 ∶ 1) and Yin Huang (0 ∶ 10) group declined,with statistical difference (P ＜ 0.05).Compared with model group,levels of TC in Yin Huang (3 ∶ 2)groulp,Yin Huang (1 ∶ 1) group,Yin Huang (1 ∶ 9) and Yin Huang (0 ∶ 10) group declined,with statistical difference (P ＜0.05).The results of pathologic morpbology,each treatment group can obviously improve the degree of liver tissue injury.Conclusion:Yin Huang combinations of different proportions can inhibit the increase of levels of ALT and AST in the serum of mice with liver injury,which has protective action for the liver injury induced by CCl and the optimal proportion is Yin Huang (1 ∶ 9).关键词
急性肝损伤/四氯化碳/茵陈/大黄/联合用药/小鼠Key words
acute liver injury/CCl/Yinchen (Herba Artemisiae Scopariae)/Dahuang (Radix et Rhizoma Rhei)/drug combination/mice分类
医药卫生引用本文复制引用
许婉琦,王奕博,郭少嫱,胡少银,王文涛,王晶..茵陈、大黄联合用药对四氯化碳肝损伤小鼠的保护作用[J].河南中医,2017,37(9):1566-1569,4.基金项目
国家级大学生科研课题招标项目(编号:201510026054) （编号:201510026054）
