军事医学2017，Vol.41Issue(7)：552-559,8.DOI:10.7644/j.issn.1674-9960.2017.07.002
微小RNA-223-3p通过调节MYH10基因促进巨核细胞多倍体化
MiR-223-3p modulates megakaryocyte polyploidization by targeting MYH10
摘要
Abstract
Objective To investigate the effect of microRNA-223-3p (miR-223-3p) on megakaryocytic differentiation and maturation, and explore the potential mechanism .Methods The endogenous expression of miR-223-3p during megakaryocyte ( MK) differentiation was detected by real-time PCR.Flow cytometry further indicated that alteration of miR-223-3p in human cell lines exerted effects on MK differentiation and maturation .By performing integrative bioinformatic analysis, the potential miR-223-3p target gene, MYH10,was identified.Real-time PCR, luciferase reporter assay and flow cytometry revealed that MYH10 was a direct target of miR-223-3p.Results Endogenous expression of miR-223-3p was in-creased with the differentiation and maturation of MK .The expression of megakaryocytic surface markers CD41 and CD61 and the ploidy were significantly increased in K562 and Meg-01 cells after transfection with miR-223-3p mimics.The expression of MYH10 decreased with the increase in miR-223-3p.Using a luciferase reporter assay ,we demonstrated that MYH10 was a direct target of MiR-223-3p.Furthermore, direct downregulation of MYH10 promoted MK polyploidization . Conclusion MiR-223-3p might regulate the polyploidization of MK by targeting MYH10.关键词
miR-223-3p/MYH10/巨核细胞/聚合酶链反应/流式细胞术Key words
miR-223-3p/MYH10/megakaryocytes/polymerase chain reaction/flow cytometry分类
生物科学引用本文复制引用
邹晓静,曲洺逸,房芳,范增,陈琳,岳文,谢小燕,裴雪涛..微小RNA-223-3p通过调节MYH10基因促进巨核细胞多倍体化[J].军事医学,2017,41(7):552-559,8.基金项目
国家863计划资助项目(2013AA020107) （2013AA020107）
国家自然科学基金资助项目(81301132) （81301132）
北京市自然科学基金资助项目(7152108) （7152108）
广州市健康医疗协同创新重大专项资助项目(201508020257,201400000003-4) （201508020257,201400000003-4）
