军事医学2017，Vol.41Issue(7)：560-566,571,8.DOI:10.7644/j.issn.1674-9960.2017.07.003
Toll样受体5对间充质干细胞生物学特性的影响
Influence of Toll-like receptor 5 on biological characteristics of mesenchymal stem cells
摘要
Abstract
Objective To investigate the effect of Toll-like receptor 5(TLR5) on the biological characteristics of human umbilical cord-derived mesenchymal stem cells (MSCs).Methods The lentiviral vectors expressing TLR5 were constructed and MSCs were infected with related lentiviruses .The overexpression of TLR5 in MSCs was verified by FACS analysis, RT-qPCR and Western blotting before its influence on cell proliferation , surface antigens and cell differentiation was evaluated.The biological function of TLR5 was verified by detecting cytokines IL-6 and IL-8 expression after TLR5 was activated by its agonist CBLB502.Results The results showed that the expression of mRNA and protein of TLR5 was upregulated in MSCs after infection of lentivirus carrying TLR5, but their proliferation , immunophenotypes and differentiation capacity were not influenced . Moreover, IL-6 and IL-8 expression was significantly increased when MSCs-TLR5 were treated with CBLB502 ( P <0.001 ).Conclusion The biological characteristics of MSCs were not affected after infection of lentivirus/TLR5, and the expressed TLR5 was functional in MSCs , which could activate the downstream signaling pathways and then regulate immune response.关键词
Toll样受体5/间充质干细胞/白细胞介素/生物学特性Key words
Toll-like receptor 5/mesenchymal stem cells/interleukin/biological characteristics分类
医药卫生引用本文复制引用
肖秀孝,薛冰华,秦亚茹,段海峰,叶绍辉..Toll样受体5对间充质干细胞生物学特性的影响[J].军事医学,2017,41(7):560-566,571,8.基金项目
国家自然科学基金资助项目(81270894) （81270894）
