军事医学2017，Vol.41Issue(7)：576-580,5.DOI:10.7644/j.issn.1674-9960.2017.07.006
ARRDC4通过NF-κB和MAPK信号通路促进EV71诱导的IL-6产生
ARRDC4 promotes EV71-triggered IL-6 production by enhancing the activation of NF-κB and MAPK signaling pathway
摘要
Abstract
Objective To study the role of arrestin domain-containing protein 4( ARRDC4) in regulation of enterovirus 71(EV71) triggered innate IL-6 production and the underlying mechanism .Methods THP-1-derived macrophages (t-M?) were transfected with ARRDC4 specific siRNA and negative control siRNA .The expression and production of IL-6, replication and virus titer of EV71, and the activation of signaling pathway adaptors were analyzed with quantitative real -time PCR, ELISA and Western blot.Results Upon EV71 infection, ARRDC4 was upregulated.ARRDC4 silencing could enhance mRNA expression and production of IL-6, thus increasing the replication and virus titer of EV71.In ARRDC4 silenced t-M?, the activation of p-65,IκBα,ERK,JNK and p38 was promoted.Conclusion ARRDC4 promotes EV71-t riggered IL-6 production by enhancing the activation of NF-κB and MAPK signaling pathway to inhibit EV71 infection, contributing to positive regulation of anti-EV71 innate immune responses .关键词
ARRDC4/NF-κB/细小RNA病毒科/肠道病毒A型,人/白细胞介素6/天然免疫/巨噬细胞/RNA,信使Key words
ARRDC4/NF-kappa B/Picornaviridae/enterovirus A/human/interleukin-6/innate immunity/macrophages/RNA/messenger分类
医药卫生引用本文复制引用
孟君,何雅青,杨洪,胡钢,程锦泉..ARRDC4通过NF-κB和MAPK信号通路促进EV71诱导的IL-6产生[J].军事医学,2017,41(7):576-580,5.基金项目
国家自然科学基金青年科学基金项目资助(81601386) （81601386）
深圳市科技计划资助项目(JCYJ20160428142411948) （JCYJ20160428142411948）
