军事医学2017，Vol.41Issue(7)：581-585,5.DOI:10.7644/j.issn.1674-9960.2017.07.007
黄芪甲苷对哮喘模型小鼠气道炎症及IL-22的影响
Astragaioside Ⅳ inhibits murine asthmatic airway inflammation through reduction of IL-22 secretion
摘要
Abstract
Objective To study the role of interleukin-22 (IL-22) in murine asthmatic airway inflammation and airway models, and explore the mechanism of astragaioside (AS) Ⅳin the treatment of asthma.Methods Ovalbumin(OVA) was used as an allergen to sensitize and challenge the mice .Thirty-two female specific-free ( SPF) four-week BALB/c mice were randomly divided into 4 groups:control group , asthma group , budesonide treatment group ( BUD group ) and AS-Ⅳgroup.HE staining and AB-PAS were used to measure the inflammation scores and goblet cells hyperplasia , enzyme-linked immunosorbent assay(ELISA) was used to analyze IL-22 levels in bronchoalveolar lavage fluid (BALF), flow cytometry was performed to analyze the proportion of Th22 cells in spleen single cell suspension , and realtime-PCR was performed to analyze the IL-22 mRNA levels in lung tissue .Results The inflammation scores of asthma group were elevated compared with the control group(P<0.05).An overall reduction of asthmatic airway inflammation was observed in the BUD group and AS-Ⅳgroup by the end of the trial .IL-22 levels in BALF and the proportion of Th22 cells in spleen single cell suspension were significantly increased after treatment in BUD and AS-Ⅳ groups(P<0.01), while the mRNA levels of IL-22 were obviously decreased(P<0.01).Conclusion The increase of IL-22 can induce airway inflammation of asthma . AS-Ⅳcan reduce Th22 cell differentiation and the expression of IL-22, thereby inhibiting the development of airway inflammation of asthma.关键词
哮喘/白细胞介素22/黄芪甲苷/气道炎症Key words
asthma/interleukin-22/astragaioside Ⅳ/airway inflammation分类
医药卫生引用本文复制引用
王明磊,王文革,张俊红..黄芪甲苷对哮喘模型小鼠气道炎症及IL-22的影响[J].军事医学,2017,41(7):581-585,5.基金项目
空军总医院基金资助项目(KZ2014005) （KZ2014005）
