军事医学2017，Vol.41Issue(7)：586-593,8.DOI:10.7644/j.issn.1674-9960.2017.07.008
在生理流动条件下分析血小板黏附聚集的简易微流控芯片技术
A simple microfluidic technology for assaying platelet adhesion and aggregation under physiological flow
摘要
Abstract
Objective To develop a simple microfluidic chip technology for analyzing platelet adhesion and aggregation under the condition of physiological flow .Methods The basic structure of the proposed microfluidic chip was a straight microchannel , where a sample pool and an outlet were located on each side respectively .The fluid dynamic behavior ,of the fluid in the microchannel was analyzed by theoretical calculation and finite element numerical analysis software ANSYS . The microchannel was first coated with type Ⅰ collagen protein.Then,blood flowed through the microchannel at 200 s-1 venous physiological shear rate and 1000 s-1 arterial physiological shear rate respectively .The behavior of the fluorescence labeled platelet adhesion and aggregation was recorded by fluorescence microscopy .Results Theoretical calculation and finite element numerical analysis showed that the microchannel with a width of 700 μm and a height of 70 μm ( aspect ration 10:1 ) had a uniform fluidic shear rate distribution .Compared with those at the 200 s-1 shear rate, the initial adhesion time, aggregation rate and the maximum surface cover rate of the platelet were significantly reduced at the 1000 s-1 shear rate ( P<0.05 ) .At the 1000 s-1 shear rate, tirofiban, an anticoagulant drug , significantly reduced the platelet aggregation in a concentration-dependent manner and the IC 50 was 23.8 nmol/L.Conclusion The technology developed in this paper can dynamically assay platelet adhesion and aggregation under the condition of physiological flow . The proposed microfluidic methods have the advantage of simple implementation and low sample cousumpation , and can be used for point-of-care detection of human platelet function , assay of the efficacy of anticoagulant drugs and for inspection of the thrombin behaviors of small animal models .关键词
微流控芯片/微通道/血栓/血小板黏附/剪切率Key words
microfluidic chip/microchannel/thrombus/platelet adhesion/shear rate分类
医药卫生引用本文复制引用
黎洋,丁玲,邓素容,杨伟,肖文海,李远..在生理流动条件下分析血小板黏附聚集的简易微流控芯片技术[J].军事医学,2017,41(7):586-593,8.基金项目
重庆市卫生局中医药科技资助项目(ZY20132132) （ZY20132132）
重庆市永川区创新能力建设平台资助项目(Ycstc2014bf5001) （Ycstc2014bf5001）
重庆市大足区科技计划资助项目(DZKJ,2014ACC1084) （DZKJ,2014ACC1084）
重庆医科大学附属永川医院院级重点研究资助项目(YJZD201302,YJZQN201534) （YJZD201302,YJZQN201534）
重庆医科大学第五临床学院大学生创新实验资助项目(201606) （201606）
