昆虫学报2017，Vol.60Issue(8)：950-961,12.DOI:10.16380/j.kcxb.2017.08.012
扬眉线蛱蝶线粒体基因组全序列测定和分析
Sequencing and analysis of the complete mitochondrial genome of Limenitis helmanni (Lepidoptera: Nymphalidae)
摘要
Abstract
[Aim] To explore the complete mitochondrial genome structure and molecular phylogenetics of Limenitis helmanni.[Methods] The complete mitochondrial genome of L.helmanni was sequenced and analyzed by using PCR and primer walking.Based on the sequences of 13 protein-coding genes and two rRNA genes of the mitochondrial genome,the phylogenetic tree of 66 lepidopteran species were constructed with Bayesian inference method.[Results] The complete mitochondrial genome of L.helmanni is a circular molecule of 15 178 bp (GenBank accession no.:KY290566),including 13 protein-coding genes,22 tRNA genes,2 rRNA genes and a 346 bp A + T-rich region.The mitogenomic gene arrangement is consistent with those of other closely related species.The mitochondrial genome of L.helmanni is biased toward a high A + T content (81.1％).All protein-coding genes start with a typical ATN initiation codon,except that COⅠ starts with the CGA codon and ND5 with the GTT codon.Most of the 13 PCGs have a complete termination codon (TAA),except COⅡ and ND4 genes which have incomplete stop codons (T).All tRNA genes show the classic clover-leaf structure,except that the dihydrouridine (DHU) arm of tRNASer(AGN) forms a simple loop.The A + T-rich region of L.helmanni contains some conserved structures such as the motif ATAGA,20 bp poly (T) stretch and some tandem repeat units,which is similar to those of other related lepidopteran species.Bayesian phylogenetic analyses supported that the relationship of Nymphalidae subfamilies is (Calinaginae + Satyrinae) +((Nymphalinae + Apaturinae) + (Heliconiinae + Limenitidinae)).[Conclusion] Limenitini is closely related with the Euthaliini,and Parthenini may be the earliest diverged lineage in Limenitidinae.The phylogenetic relationship of Limenitidinae species based on the mitochondrial genome is not consistent with the results of the traditional morphology-based taxonomy.关键词
鳞翅目/蛱蝶科/扬眉线蛱蝶/线粒体基因组/系统发育Key words
Lepidoptera/Nymphalidae/Limenitis helmanni/mitochondrial genome/phylogeny分类
生物科学引用本文复制引用
王菊平,曹天文,张越,范仁俊,张苗,石保明,彭福成..扬眉线蛱蝶线粒体基因组全序列测定和分析[J].昆虫学报,2017,60(8):950-961,12.基金项目
山西省农业科学院农业科技创新研究项目(ZDSYS1509) （ZDSYS1509）
山西省应用基础研究项目(201601D202059,2011011033-2) （201601D202059,2011011033-2）
山西省科技重大专项(20121101007) （20121101007）
