南京林业大学学报（自然科学版）2017，Vol.41Issue(5)：1-6,6.DOI:10.3969/j.issn.1000-2006.201606014
模拟氮沉降对杨树人工林土壤微生物群落碳源利用类型的影响
Effects of nitrogen deposition on soil microbial community C?source metabolism of poplar plantation
摘要
Abstract
[Objective]Study the effects of elevated nitrogen deposition on soil microbial community structure in a poplar plantation.[Method] The field experiment was designed at Dongtai Forestry Farm in Jiangsu Province, a coastal area of eastern China in June 2015, and we used Biolog ECO method and conducted nitrogen addition at four levels: N0 (0 kg/(hm2·a)), N1 (50 kg/(hm2·a)), N2 (100 kg/(hm2·a)), and N3 (150 kg/(hm2·a)). [Result]The soil microbial C?source metabolic capacity was improved under N2 treatment, but was inhibited under higher levels of nitro?gen deposition. Utilization of phenols and amines were the highest in N3 and N1, respectively. Average well color devel?opment( AWCD) and the Shannon diversity index were both significantly and positively correlated with nitrate nitrogen content ( P<005) , indicating that the C?source metabolism and community structure were influenced significantly by ni?trate nitrogen content. The PC1 and PC2 in a principal component analysis ( PCA) accounted for 5004% and 2502%, respectively, of the variation in microbial community metabolic diversity caused by nitrogen deposition. The PC1 was cor?related with carbohydrates (-0869), phenols (-0780), amino acids (0702) and carboxylic acids (0821). The PC2 was correlated with polymers (-0688) and amines (0802).[Conclusion]After two years of nitrogen addition during May-October each year, the nitrogen amendment significantly changed the types of carbon utilized by the micro?bial community. There were significantly positive correlations between the contents of soil nitrate nitrogen and bacterial carbon metabolism levels as well as the diversity indices. The results of PCA indicated that the dominant carbon sources were carbohydrates and carboxylic acid.关键词
氮沉降/土壤微生物/碳源代谢/群落功能多样性/杨树人工林Key words
nitrogen deposition/soil microbial/C⁃source metabolism/functional diversity of community/poplar planta⁃tion分类
农业科技引用本文复制引用
马慧君,张雅坤,许文欢,葛之葳,阮宏华..模拟氮沉降对杨树人工林土壤微生物群落碳源利用类型的影响[J].南京林业大学学报（自然科学版）,2017,41(5):1-6,6.基金项目
国家重点基础研究发展计划(2012CB416904) （2012CB416904）
江苏省基础研究计划(自然科学基金)青年基金项目(BK20130973) （自然科学基金）
江苏高校优势学科建设工程资助项目( PAPD) （ PAPD）
