南京林业大学学报（自然科学版）2017，Vol.41Issue(5)：18-26,9.DOI:10.3969/j.issn.1000-2006.201609042
原始红松林土壤理化及微生物碳代谢特征对生长季动态的响应
Responses of soil physicochemical properties and soil microorganism characteristics regareding as carbon metabolism in original Korean pine forest
摘要
Abstract
[Objective]Understand the seasonal variations and mechanisms of the differences in the soil microbial com?munity and soil physicochemical properties for a original Korean pine forest, and explore the seasonal changes in the soil physicochemical properties in response to meteorological factors.[Method]The study site was located in Xiaoxing′an Mountain, a typical original broadleaf Korean pine forest, and the soil ( 0-10 cm and≥10-20 cm) was sampled from May to October in 2015. The soil microbial functional diversity and soil physicochemical properties were measured by using the Biolog microplate method and conventional measurement methods. [Result] The soil physicochemical properties had significant differences in different months. The average well color development ( increased of AWCD) val?ue gradually with prolonged culture time;the increase of AWCD value gradually weakened after 168 hours. The AWCD values and microbial diversity were significantly different among months. The metabolic activity of the soil microbial com?munity had significant seasonal variation, and was the lowest in July and the highest in June ( P< 0.05) . Amino acids, polymers, and carbohydrates were the dominant carbon sources. The results of principal component analysis ( PCA)showed that the metabolic characteristics of soil microbial community carbon were divided into three clusters: May to June, July, and from August to October. The utilization of carbohydrates, amino acids and carboxylic acids were more sensitive to seasonal variations than that use of other carbon sources. The results of correlation analysis showed that AWCD value and microbial diversity indices had significant positive correlations with available P, available K, soil water content, and available N. Soil water content ( which explained 12.76% of the variance) , available P ( which explained 30.71%) were the main factors that affected the seasonal variation in microbial functional diversity according to variation partition analysis.[Conclusion]The soil physicochemical properties and microbial functional carbon metabolism charac?teristics were significantly different during the growth season and the non?growth season in the original broadleaf Korean pine forest, and this was most significantly influenced by different precipitation in different months.关键词
原始红松林/土壤微生物/Biolog/碳代谢/降水Key words
original Korean pine forest/soil microorganism/Biolog/carbon metabolism/precipitation分类
生物科学引用本文复制引用
孙雪,韩冬雪,刘岩,冯富娟,隋心..原始红松林土壤理化及微生物碳代谢特征对生长季动态的响应[J].南京林业大学学报（自然科学版）,2017,41(5):18-26,9.基金项目
国家自然科学基金项目(31670496) （31670496）
中央高校基本科研业务费专项资金项目(2572015EA02) （2572015EA02）
