南京林业大学学报（自然科学版）2017，Vol.41Issue(5)：42-48,7.DOI:10.3969/j.issn.1000-2006.201601006
矢竹地下茎转录组测序及节间生长相关基因表达分析
Transcriptome sequencing, de novo assembly and expression analysis of several genes related to internode development in Pseudosasa japonica
摘要
Abstract
[Objective]Describe the molecular basis of bamboo rhizome development. [Methods] We used next?genera?tion sequencing technology and qRT?PCR to analyze the transcriptome profile of the underground stems of Pseudosasa ja?ponica and the expression pattern of genes related to internode elongation, respectively. [Results] A total of 119767 unigenes, with an average length of 1008 bp were determined, 63.8% of which had annotated results. A total of 16254 unigenes were annotated to 137 KEGG( kyo to Encyclopedia of Genes and Genomes) pathways. MapMan software identi?fied 1864 transcription factors, 603 hormone related genes, and 564 cell?wall building related genes, including 92 uni?genes related to lignin biosynthesis. The qRT?PCR analysis showed that, among the nine genes used for detecting, gib?berellin?related genes had the highest expression level in the elongation initiation stage of internode development, whereas cell wall growth? and cytoskeleton?related genes had the strongest expression in the rapid elongation of inter?nodes. [Conclusion] These results illustrate the molecular basis for rhizome development in Ps. japonica. The molecular mechanism underlying the development of rhizomes is a complex network consisting of hormones, transcription factors and downstream functional genes.关键词
矢竹/转录组/基因表达/赤霉素/细胞壁/节间生长/高生长Key words
Pseudosasa japonica/transcriptome/gene expression/gibberellin/cell wall/internode development/rapid growth分类
农业科技引用本文复制引用
魏强,丁雨龙..矢竹地下茎转录组测序及节间生长相关基因表达分析[J].南京林业大学学报（自然科学版）,2017,41(5):42-48,7.基金项目
国家林业公益性行业科研专项项目(201504106) （201504106）
国家自然科学基金项目(31301808,31670602) （31301808,31670602）
江苏高校优势学科建设工程资助项目 ( PAPD) （ PAPD）
