南京医科大学学报（自然科学版）2017，Vol.37Issue(9)：1124-1130,7.DOI:10.7655/NYDXBNS20170911
橄榄苦苷抑制丙烯醛诱导的HBE细胞内质网应激的机制研究
Mechanism study of oleuropein inhibiting endoplasmic reticulum stress induced by acrolein in HBE cells
摘要
Abstract
Objective:To study the effect of oleuropein in combination of acrolein on proliferation and apoptosis related to endoplasmic reticulum stress(ERS) in human bronchial epithelial cells.Methods:HBE cells were treated with oleuropein and acrolein.MTT assay was performed to detect cell viability.Plate clone formation assay was used to get colony forming efficiency.Hoechst 33258 staining and flow cytometric assay were applied to investigate the apoptosis of cells.The levels of ERS biomarkers including glucose-regulated protein 78(GRP78) and C/EBP homologous protein(CHOP) were measured by Western blot.The mRNA expressions of GRP78 and CHOP were analyzed by real-time PCR.Twelve male Sprague-Dawley rats were treated with acrolein alone through intraperitoneal injection,or olive leaf extract in combination through gavage.Lung tissues were obtained from the rats for detecting the expressions of GRP78 and CHOP.Results:HBE cell proliferation was inhibited by acrolein in a dose dependent manner.Cell proliferation increased significantly with the combination of oleuropein.Oleuropein combined with acrolein also inhibited acrolein-induced apoptosis in HBE cells.Western blot demonstrated that ERS biomarkers such as GRP78 and CHOP were induced by acrolein combined with oleuropein,acrolein-induced ERS was alleviated.However,oleuropein had no effects on the GRP78 and CHOP mRNA expression.The in vivo results of Sprague-Dawley rats were in agreement with those in vitro.Conclusion:Acrolein could trigger ERS in HBE cells and lead to cell apoptosis.Nevertheless,oleuropein in combination with acrolein could inhibit the acrolein-induced apoptosis.The potential mechanism may be related with the alleviation of acrolein-induced ERS.In protein translational level,oleuropein can inhibit apoptosis induced by acrolein via ERS pathway.关键词
橄榄苦苷/丙烯醛/HBE细胞/内质网应激Key words
oleuropein/acrolein/HBE cells/endoplasmic reticulum stress分类
医药卫生引用本文复制引用
徐朝琪,许玉宇,姜盼,冯晴..橄榄苦苷抑制丙烯醛诱导的HBE细胞内质网应激的机制研究[J].南京医科大学学报（自然科学版）,2017,37(9):1124-1130,7.基金项目
国家自然科学基金(81472977) （81472977）
江苏高校优势学科建设工程资助项目 （）
