南京医科大学学报（自然科学版）2017，Vol.37Issue(9)：1131-1135,1181,6.DOI:10.7655/NYDXBNS20170912
甲基苯丙胺对大鼠小胶质细胞的损伤及炎性相关基因表达的影响
Damage of cells and effects of inflammatory related genes induced by methamphetamine in rat microglial cells
摘要
Abstract
Objective:To explore the effects of cell injury and the expression of inflammation related genes in rat microglial cells induced by methamphetamine(Meth).Methods:Cell viability and apoptosis induced by Meth were detected by MTF and in situ end labeling(TUNEL) after primary culture of SD fetal rat microglial cells.The effect of Meth on the expression of inflammatory cytokines mRNA in the microglial cells was evaluated by real time fluorescence quantitative polymerase chain reaction(real-time PCR).The secretion of interleukin(IL)-6,tumor necrosis factor(TNF)-α and inducible nitric oxide synthase(iNOS)in microglia cells induced by Meth were detected by ELISA method.Results:MTIT showed that Meth reduced microglia viability in a concentration dependent manner (25,50,100,and 200 μ mol/L) with statistically significant at a concentration of 200 μmol/L (P＜0.05).TUNEL results showed that 200 μ mol/L Meth significantly contributed to cell apoptosis compared with the control group(P＜0.05).Q-PCR results showed that Meth reduced the expression levels of IL-24 and nitric oxide synthase 3(NOS3) in microglial cells for 24 hours.However,the expression levels of Peli3,Sigma receptor 1(sigl)-R,IL-1,IL-6 and Toll like receptor 4(TLR4) were up-regulated.In addition,protein levels were also confirmed that Meth promoted the secretion of IL-6 and TNF-α.Conclusion:Meth exposure obviously contributes to microglial damage.Moreover,it causes IL-1 β,IL-1 R,IL-6,TLR4 and other inflammatory factors mRNA expression changes,and promotes the secretion of IL-6 and TNF-α,which may damage the central nervous system,resulting in neurotoxicity.关键词
甲基苯丙胺/小胶质细胞/炎性因子Key words
methamphetamine/microglial cells/inflammatory factor分类
医药卫生引用本文复制引用
程洁,蒋雷,环飞,黄雨蒙,王军..甲基苯丙胺对大鼠小胶质细胞的损伤及炎性相关基因表达的影响[J].南京医科大学学报（自然科学版）,2017,37(9):1131-1135,1181,6.基金项目
国家自然科学基金(81673213,81202230) （81673213,81202230）
江苏省自然科学基金(BK20151557) （BK20151557）
江苏高校优势学科建设工程资助项目 （）
