山东医药2017，Vol.57Issue(35)：22-25,4.DOI:10.3969/j.issn.1002-266X.2017.35.007
Cyclin D1 siRNA转染人肝癌细胞的增殖、凋亡变化及其机制
Changes in proliferation and apoptosis of human hepatocellular carcinoma cells transfected with Cyclin D1 siRNA
摘要
Abstract
Objective To observe the changes of proliferation and apoptosis of human hepatocellular cells transfected with Cyclin D1 siRNA,and to explore the mechanism. Methods The cultured human hepatoma cell line Hep3B was di-vided into the blank control group,negative control group,and experimental group. The negative control group and the ex-perimental group were transfected with NC siRNA and Cyclin D1 siRNA by liposomes,and the cells were harvested after 48 h. MTT was used to detect cell proliferation;the flow cytometry was used to observe apoptotic cells and we calculated apop-tosis rate;the Caspase-3 protein in cells of each group was detected by immunohistochemistry;the mRNA and protein ex-pression levels of hepatitis B virus x protein (HBx),xeroderma pigmentosum D (XPD),Bcl-2,and Bax in cells of each group were detected by RT-PCR and Western blotting,respectively. Results Compared with the blank control group and the negative control group,the cell proliferation ability of the experimental group decreased,the apoptosis rate increased, and the relative expression of Caspase-3 increased (all P < 0. 01). Compared with the blank control group and the negative control group,the mRNA and protein expression levels of HBx and Bcl-2 in the experimental group were down-regulated, while those of XPD and Bax were up-regulated (all P < 0. 01). Conclusion The proliferation of human hepatoma cells transfected with Cyclin D1 siRNA is inhibited and the apoptosis increases,and the mechanism may be related to the down-regulation of HBx and Bcl-2 expression,and up-regulation of XPD and Bax expression.关键词
细胞周期蛋白D1/肝细胞癌/细胞增殖/细胞凋亡/人剪切修复基因Key words
Cyclin D1/hepatocellular carcinoma/cell proliferation/apoptosis/human shear repair genes分类
医药卫生引用本文复制引用
张弘英,胡利琳,元文峰,丁浩..Cyclin D1 siRNA转染人肝癌细胞的增殖、凋亡变化及其机制[J].山东医药,2017,57(35):22-25,4.基金项目
国家自然科学基金资助项目(81300348) （81300348）
江西省自然科学基金资助项目(20151BAB205100). （20151BAB205100）
