山东医药2017，Vol.57Issue(35)：29-32,4.DOI:10.3969/j.issn.1002-266X.2017.35.009
卵巢癌HELQ蛋白结构和功能的生物信息学工具分析预测
Bioinformatics analysis and prediction of HELQ protein structure and function
摘要
Abstract
Objective To predict the structure and function of ovarian cancer anti-tumor gene HELQ protein. Meth-ods The tools of ExPASy Server were employed to analyze the physicochemical properties of HELQ protein. Its subcellu-lar localization and transmembrane topology were predicted by PSORTⅡand TMHMM,respectively. The signal peptide of HELQ was predicted by SignalP 4. 0. ScanProsite and NCBI conserved domain search data were used to analyze the function domain and conserved domain. PSIPRED and I-TASSER were employed to construct the secondary structure and three-di-mensional model and find the ligand binding sites of HELQ. STRING 10. 0 was used to predict the interaction of HELQ with other proteins. Results The HELQ protein was mainly located in the nucleus (47. 8%)and cytoplasm (39. 1%). HELQ had 4 conserved domains,consisting of DEAD,HELICc,HHH_5,and PRK02362,and contained 2 function do-mains:Helicase_ATP-Bind_1 and Helicase_Cter. The secondary structure and three-dimensional model were constructed. In the secondary structure,HELQ was composed of α helix (54%),random coils (31%),and 10. 26% β-structure. The results of three-dimensional model found that HELQ had a enzyme activity site His341 and the ligand binding sites appeared in Ile333,Lys335,Tyr337,Gln340,Pro360,Thr361,Ser362,Gly363,Gly364,Lys365,Thr366,Leu367,Glu464 and Ala711 which combined with ATP. The result of cross-linked protein analysis discovered that HELQ might be interaction with RAD51,POLA1,PLON,FANCD2,and DHX8. Conclusion HELQ protein may have ATP-dependent helicase structure and function,and participate in the DNA damage repair process.关键词
卵巢癌/HELQ基因/HELQ蛋白/三级结构/ATP依赖性解旋酶Key words
ovarian carcinoma/HELQ gene/HELQ protein/tertiary structure/ATP-dependent helicase分类
医药卫生引用本文复制引用
李亚萍,邹余粮,张琳,黄新,秦勇..卵巢癌HELQ蛋白结构和功能的生物信息学工具分析预测[J].山东医药,2017,57(35):29-32,4.基金项目
陕西省科学技术研究发展计划项目(2013JM4064). （2013JM4064）
