生命科学研究2017，Vol.21Issue(4)：295-301,7.DOI:10.16605/j.cnki.1007-7847.2017.04.003
翘嘴鳜性腺型芳香化酶基因CYP19a的克隆及表达研究
Cloning and Expression Analysis of CYP19a Gene in Mandarin Fish Siniperca chuatsi
摘要
Abstract
By using the homology-based cloning combined with rapid-amplification of cDNA ends (RACE),the full length cDNA sequence of gonad-specific aromatase (P450aromA) gene CYP19a from Mandarin fish (Siniperca chuatsi) was obtained.The real-time quantitative PCR (qPCR) was used to detect the relative mRNA expression levels of CYP19a in different tissues and gonads at different development periods.The data showed that the full length of goldfish CYP19a cDNA was 1 873 bp (GenBank accession number KX911988),with an open reading frame of 1 581 bp encoding a protein of 526 amino acids,which contains the transmembrane helix region,I-helix region,Ozol's peptide region,aromatase-specific conserved region and heme-binding region.Meanwhile,homologous analysis of CYP19a sequences revealed that the gene was more conservative in vertebrates.In addition,the qPCR analysis showed that CYP19a transcripts were highly abundant in ovary and testis (P＜0.05),but less abundant in brain and liver.The relative mRNA expression level of CYP19a in pre-reproductive period of gonad was significantly higher than that in reproductive period (P＜0.05).Taken together,the results indicate that CYP19a plays an important role in fish gonad formation and developmental processes.关键词
翘嘴鳜/性腺型芳香化酶基因(CYP19a)/基因克隆与表达/序列比对/同源性分析Key words
Mandarin fish (Siniperca chuatsi)/gonad-specific aromatase gene (CYP19a)/gene cloning and expression/sequence alignment/homologous analysis分类
生物科学引用本文复制引用
邹立军,龚婧,吉璐,庄远红,廖海艳,黄文..翘嘴鳜性腺型芳香化酶基因CYP19a的克隆及表达研究[J].生命科学研究,2017,21(4):295-301,7.基金项目
湖南省教育厅科学研究项目(14C0252) （14C0252）
湖南省科技计划项目(2014FJ4261) （2014FJ4261）
