生命科学研究2017，Vol.21Issue(4)：312-317,6.DOI:10.16605/j.cnki.1007-7847.2017.04.006
病毒感染复数影响腺病毒感染T淋巴细胞效率的初步研究
Influence of Multiplicity of Infection on the Efficiency of Adenovirus Infection in T Lymphocytes
摘要
Abstract
Previous studies have found that increasing multiplicity of infection (MOI) can effectively improve the efficiency of adenoviruse serotype 5 (Ad5) infection in T cells;however,little is known about the underlying mechanism.Here,Jurkat T cells were selected as target cells,and the effects of different MOI on the viral binding and viral entry in the replication cycle of Ad5-GFP were investigated by means of quantitative fluorescence PCR and transmission electron microscopy.It was found that high MOI significantly increased the number of adenovirus into the T cell during the endocytosis process,but not the number of adenovirus adsorbed on T cells.These results indicate that increase of MOI is beneficial to the adenovirus into the T cells by endocytosis,improving the efficiency of adenovirus infection in T lymphocytes,and this provide a theoretical basis for further study of the adenovirus infection mechanism in T lymphocytes.关键词
腺病毒/感染复数(MOI)/感染效率/病毒胞吞/T淋巴细胞Key words
adenovirus/multiplicity of infection (MOI)/efficiency of infection/endocytosis/T lymphocytes分类
生物科学引用本文复制引用
张琼宇,马珊珊,唐小标,葵旭..病毒感染复数影响腺病毒感染T淋巴细胞效率的初步研究[J].生命科学研究,2017,21(4):312-317,6.基金项目
2013年度湖南省教育厅科学研究面上项目(13C970) （13C970）
永州市2015年度第二批指导性科技计划项目(永科发[2015]10号) （永科发[2015]10号）
