生命科学研究2017，Vol.21Issue(4)：318-324,7.DOI:10.16605/j.cnki.1007-7847.2017.04.007
鱼腥藻PCC7120中琥珀酸半醛脱氢酶的初步表征及结构分析
Preliminary Characterization and Structural Analysis of Succinic Semialdehyde Dehydrogenase from Anabaena sp.PCC7120
摘要
Abstract
Succinic semialdehyde dehydrogenases (SSADHs) of cyanobacteria catalyze the oxidation of succinic semialdehyde to succinic acid and complete the cyanobacterial TCA cycle.The all3556 gene of A nabaena sp.PCC7120 was predicted to encode a succinic semialdehyde dehydrogenase by BLAST analysis.To prove the catalytic function of the protein encoded by all3556 gene,the plasmid pET28a-all3556 was constructed and transformed into E.coli BL21(DE3) competent cells.Then the all3556 protein was purified by Ni-NTA affinity chromatography.Steady-state kinetic studies showed that all3556 was a NADP+-dependent succinic semialdehyde dehydrogenase.Bioinformatic analysis indicated that all3556 protein and other succinic semialdehyde dehydrogenases had identities in amino acid sequences and that the residues in the catalytic center were highly conserved in different succinic semialdehyde dehydrogenases.关键词
鱼腥藻PCC7120/琥珀酸半醛脱氢酶(SSADH)/重组表达/蛋白质纯化及表征/结构分析Key words
A nabaena sp.PCC7120/succinic semialdehyde dehydrogenase (SSADH)/recombinant expression/protein purification and characterization/structural analysis分类
生物科学引用本文复制引用
王小琴,李至敏,雷国风,汤亚兰,李志敏..鱼腥藻PCC7120中琥珀酸半醛脱氢酶的初步表征及结构分析[J].生命科学研究,2017,21(4):318-324,7.基金项目
国家自然科学基金资助项目(31400054,31560250) （31400054,31560250）
江西省青年科学基金重大项目(20161ACB21012) （20161ACB21012）
教育部留学回国人员科研启动基金项目(第46批) （第46批）
