天津中医药大学学报2017，Vol.36Issue(5)：374-377,4.DOI:10.11656/j.issn.1673-9043.2017.05.14
合煎与单煎对四物汤中4种化学成分溶出量的影响
Effect of four chemical components dissolution in Siwu decoction by decocting together and single
摘要
Abstract
[Objective] To establish a method for content determination of peoniflorin, ferulic acid, ligustilide and senkyunolide I in Siwu decoction and study the dissolution variation between mixed-decoction and single-decoction. [Methods] RP-HPLC with Cosmosil C18 (4.6 mm ×250 mm, 5 μm) was used, methanol-0.3% formic acid solution gradient elution was applied. The flow rate was 1.0 mL/min. The detection wavelength was 280 nm and the column temperature was 30 ℃. The dissolution variation was analyzed between mixed-decoction and single-decoction. [Results] The linear range of four components were 19.38~310.00μg/mL (r=0.9994), 1.51~24.16μg/mL (r=0.9994), 1.50~24.00μg/mL (r=0.9996) and 2.00~32.00μg/mL (r=0.9994) with average recovery 95.89%(RSD=2.90%), 97.08%(RSD=2.28%), 96.84%(RSD=2.87%) and 98.77%(RSD=1.98%) respectively. The dissolution of senkyunolide I was lower mixed-decoction than single-decoction of Angelica and Chuanxiong. The dissolution of ferulic acid and ligustilide were higher mixed-decoction than single-decoction of Angelica and Chuanxiong. The dissolution of peoniflorin was higher mixed-decoction than single-decoction of Radix paeoniae Alba. [Conclusion] The dissolution of chemical compositions were not a simple superposition of their dissolution in single medicine, but have their own inherent compatibility regularity. This research from the perspective of the chemical composition dissolution provides a reference for the study of Siwu decoction .关键词
四物汤/合煎/单煎/芍药苷/阿魏酸/藁本内酯/洋川芎内酯IKey words
Siwu decoction/mixed-decoction/single-decoction/peoniflorin/ferulic acid/ligustilide/senkyunolide I分类
医药卫生引用本文复制引用
郭江玉,孙磊,闫彦,余河水,李薇,张丽娟,宋新波..合煎与单煎对四物汤中4种化学成分溶出量的影响[J].天津中医药大学学报,2017,36(5):374-377,4.基金项目
国家科学技术重大专项基金项目(2013ZX095081050010) （2013ZX095081050010）
天津市科学技术计划项目(14TXZYJC00440). （14TXZYJC00440）
