医药导报2017，Vol.36Issue(10)：1103-1107,5.DOI:10.3870/j.issn.1004-0781.2017.10.004
Orexin-1受体抑制剂对大鼠骨髓间充质干细胞增殖和成骨分化的影响
Effects of Orexin-1 Receptor Inhibitor on Proliferation and Osteogenic Differentiation of Bone Marrow Mesenchymal Stem Cells
摘要
Abstract
Objective To observe the effects of orexin-1 receptor inhibitor on proliferation and estrogenic differentiation of bone marrow mesenchymal stem cells ( BMSCs) . Methods BMSCs were isolated and cultured from Sprague-Dawley rats, and orexin-1 receptor inhibitor was prepared to different concentration of solutions:10-2 , 10-3 , 10-4 , 10-5 and 10-6 mol·L-1 . Then, the cells at passage 3 were cultured in orexin-1 receptor inhibitor solutions. The cell growth, alkaline phosphatase activity and osteocalcin secretion were measured by MTT, PNPP and ELISA, respectively. The mRNA expression levels of Runx2 and COL1A1 were detected by real-time quantitative PCR. Results The best concentration of orexin-1 receptor inhibitor for the proliferation of BMSCs was 1 × 10-4 mol·L-1 . Orexin-1 receptor inhibitor promoted the proliferation at concentration of 10-6 , 10-5 , 10-4 mol·L-1 in 3, 5 and 7 days. Orexin-1 receptor inhibitor at concentration of 10-6 , 10-5 and 10-4 mol·L-1 for 5 days significantly stimulated ALP activity. Orexin-1 receptor inhibitor significantly up-regulated Runx2 and COL1A1 mRNA expression at concentration of 10-6, 10-5, 10-4 mol·L-1 for 5 days (P<0.05). Conclusion Orexin-1 receptor inhibitor can promote BMSCs proliferation and stimulated ALP activity and osteocalcin secretion.关键词
orexin-1受体抑制剂/骨髓间充质干细胞/碱性磷酸酶/骨钙素Key words
Orexin-1 receptor inhibitor/Bone marrow mesenchymal stem cell/Alkaline phosphatase/Osteocalcin分类
医药卫生引用本文复制引用
张丽媛,纳青青,吴天秀..Orexin-1受体抑制剂对大鼠骨髓间充质干细胞增殖和成骨分化的影响[J].医药导报,2017,36(10):1103-1107,5.基金项目
年广东医学院科研基金资助项目(2XJ1401P) （2XJ1401P）
2015 年湛江市非资助科技攻关计划项目( 2015B01083) （ 2015B01083）
2015年湛江市财政资金科技专项竞争性分配项目( 2015A01030) （ 2015A01030）
