医药导报2017，Vol.36Issue(10)：1179-1182,4.DOI:10.3870/j.issn.1004-0781.2017.10.022
高效液相色谱法测定异甘草酸镁中18β-异构体的含量
Determination of 18β-isomer in Magnesium Isoglycyrrhizinate by HPLC
摘要
Abstract
Objective To establish a HPLC method for determination of 18β-isomer in magnesium isoglycyrrhizinate. Methods The determination was performed by Agilent Extend-C18 column ( 4. 6 mm × 250 mm, 5 μm ) . Mobile phase consisted of 0. 1 mol·L-1 potassium dihydrogen phosphate buffer solution ( adjusted to pH 7. 0 with potassium hydroxide )-acetonitrile (80︰20) at the flow rate of 1.0 mL·min-1. The column temperature was 30 ℃, and the detection wavelength was set at 250 nm. Results The resolution of magnesium isoglycyrrhizinate and 18β-isomer was greater than 2.0. The linear range of them was 0.41-2.46μg·mL-1( r=0.9998) , the detection limit was 0.21 ng, and the average recovery were 100.2%,99.1%, 110.2%,RSD were 0.9%,0.1%,0.2%(n=3). Conclusion The method is simple and accurate, and can be used for determination of 18β-isomer in magnesium isoglycyrrhizinate.关键词
异甘草酸镁/18β-异构体/色谱法,高效液相/含量测定Key words
Magnesium isoglycyrrhizinate/18β-isomer/Chromatography,high performance liquid/Content determination分类
医药卫生引用本文复制引用
王善春,顾红梅,张喜全,陈智林,李洋,李军..高效液相色谱法测定异甘草酸镁中18β-异构体的含量[J].医药导报,2017,36(10):1179-1182,4.基金项目
国家"重大新药创制" 科技重大专项( 2008ZX09201-003) （ 2008ZX09201-003）
