诊断学理论与实践2017，Vol.16Issue(4)：377-383,7.DOI:10.16150/j.1671-2870.2017.04.007
Klotho在高磷诱导的血管平滑肌细胞钙化中的表达研究
Klotho expression in hyperphosphate-induced calcified vascular smooth muscle cells
摘要
Abstract
Objective:To investigate the changes of Klotho expression in rat vascular smooth muscle cells (VSMCs)calcification induced by high phosphate.Methods:Rat VSMCs was cultured in vitro,the proliferation of VSMCs induced by different concentrations of phosphate was detected by cell counting kit-8 (CCK-8).VSMCs were treated with high phosphate (3 mmol/L) for 3 days or 6 days,and the calcification of VSMCs was evaluated by alizarin red staining.The activity of alkaline phosphatase (ALP) and calcium content in VSMCs were detected by ALP assay kit and calcium colorimetric assay kit,respectively.The expression of mRNA and protein of bone morphogenetic protein 2 (BMP-2),Runt related transcription factor 2 (Runx2) and Klotho were detected by quantitative real-time polymerase chain reaction (qrt-PCR) and Western blotting (WB).Results:Higher-phosphate media inhibited the proliferation of VSMCs.After being incubated in high-phosphate media for 6 days,calcified nodules were observed with alizarin red staining and calcium content was significantly increased;qrt-PCR demonstrated that levels of BMP-2 and Runx2 mRNA and protein were increased and the expressions of Klotho mRNA and protein levels were decreased.Conclusions:Klotho might be involved in the hyperphosphate-induced calcification and phenotype changes in VSMCs.关键词
血管平滑肌细胞/Klotho/高磷/钙化Key words
Vascular smooth muscle cells/Klotho/High phosphate/Calcification分类
医药卫生引用本文复制引用
蒋钻红,陈孜瑾,汪知玉,陈秋静,陆林,马晓波,俞海瑾,陈晓农..Klotho在高磷诱导的血管平滑肌细胞钙化中的表达研究[J].诊断学理论与实践,2017,16(4):377-383,7.基金项目
国家自然科学基金(81600590) （81600590）
上海市自然科学基金(14ZR1425400) （14ZR1425400）
上海市卫生计划生育委员会课题(20154Y0015) （20154Y0015）
