中国感染控制杂志2017，Vol.16Issue(10)：916-919,930,5.DOI:10.3969/j.issn.1671-9638.2017.10.005
肺炎链球菌疫苗抗原促进CRTH2(CD4+CD294+Th2)细胞增殖的实验研究
Pneumococcal vaccine promotes the proliferation of CRTH2 (CD4+CD294+ Th2)cells
摘要
Abstract
Objective To study the proliferation of CRTH2 (CD4+ CD294+ Th2)cells promoted by pneumococcal vaccine through antigen presentation of dendritic cells (DCs),so as to provide a new approach for amplification and sorting of Th2 cells.Methods CDs induced from peripheral blood mononuclear cells were cocultured with T lym-phocytes after loading pneumococcal vaccine antigen,mixed lymphocyte reaction (MLR)was detected by cell count-ing kit-8(CCK8),DCs and CRTH2 cells were analyzed by flow cytometry.Results Pneumococcal vaccine could promote the maturation of DCs,together with TNF-a,it was adjuvant for maturation of DCs.Pneumococcal vaccine antigen-loaded DCs could increase the rate of subsets of CRTH2 cells on day 5([0.93±0.10]%)compared with day 1([0.70±0.02]%),and absolute number also increased (both P <0.05).Conclusion Amplification of CRTH2 cells can be greatly promoted by pneumococcal vaccine antigen-loaded DCs,which might be one of the effective way to induce amplification of Th2 cells.关键词
肺炎链球菌疫苗/树突状细胞/CRTH2细胞/细胞增殖Key words
pneumococcal vaccine/dendritic cell/CRTH2 cell/cell proliferation分类
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唐玫琴,田发青,李举亨,黄映彩,李惠卿,成小慧..肺炎链球菌疫苗抗原促进CRTH2(CD4+CD294+Th2)细胞增殖的实验研究[J].中国感染控制杂志,2017,16(10):916-919,930,5.基金项目
深圳市科技创新项目科技研发基金(JCYI 20140414123738256) （JCYI 20140414123738256）
深圳市龙岗区科技发展基金(YLWS 20150514150041453) （YLWS 20150514150041453）
