中国临床药理学与治疗学2017，Vol.22Issue(8)：841-845,5.
信号蛋白HMGB1和RAGE在Aβ25-35损伤神经元中的表达变化
Changes of signal protein HMGB1 and RAGE expression in neurons damaged by Aβ25-35
摘要
Abstract
AIM:To investigate the changes of high mobility group box 1 protein (HMGB1) and receptor of advanced glycation (RAGE) in neurons damaged by β-amyloid 25-35 (Aβ25-35).METHODS:Primary hippocampal neurons were chosen and cultured 7 days with different concentrations of Aβ25-35.CCK-8 test was used to detect cell viability for determining drug concentration and reaction time.Primary hippocampal neurons were then divided into two groups,i.e.the normal cell group (group C) and the injury group (group I).Neuronal morphology and Western blot technique were applied to detect the expression level of HMGB1 in cytoplasm and nucleus as well as RAGE and NF-κB in the cytoplasm.The quantity of HMGB1,IL-1β and TNF-α in supernatant of neurons was detected by ELISA.RESULTS:Neuron injured model was determined with Aβ25-35 concentration at 25 μmol/L and reaction time for 24 h by CCK-8 test.Morphology showed neurons decreased significantly and most of them in aggregation state in group I.Western blot showed the expression level of HMGB1 in cytoplasm was significantly lower than that in group C (1.596 ± 0.189 vs.0.146 ± 0.043,P ＜ 0.05),while HMGB1 in cell nucleus was higher than that in groupC (0.934±0.145 vs.1.370±0.354,P＜ 0.05),the expression level of RAGE and NF-κB in cytoplasm were higher in group I than those in group C (0.962 ±0.180 vs.1.253 ±0.254,0.825 ± 0.116 vs.1.023 ± 0.150,P ＜ 0.05).ELISA showed the quantity of HMGB1,IL-1β3 and TNF-α in supernatant were significantly higher in group Ⅰ than those in group C (P ＜0.05).CONCLUSION:Signal proteins of HMGB1,RAGE and inflammatory factors such as IL-1 β and TNF-α were obviously increased in Aβ25-35 injured neurons;HMGB1 might be involved in Aβ25-35 injured neuronal inflammation through RAGE/ NF-κB inflammatory pathway.关键词
阿尔茨海默病/淀粉样蛋白/神经元/高迁移率族蛋白1Key words
Alzheimer's disease/β-amyloid protein/neuron/high mobility group protein 1分类
医药卫生引用本文复制引用
杨芳骅,南克,项芳芳,刘绪华,韩园,曹红,李军..信号蛋白HMGB1和RAGE在Aβ25-35损伤神经元中的表达变化[J].中国临床药理学与治疗学,2017,22(8):841-845,5.基金项目
国家自然科学基金(81271204) （81271204）
浙江省科技厅公益项目(2016C37098) （2016C37098）
浙江省自然科学基金(LY17H090015) （LY17H090015）
